PUBLICATION

One for all-a highly efficient and versatile method for fluorescent immunostaining in fish embryos

Authors
Inoue, D., and Wittbrodt, J.
ID
ZDB-PUB-110603-4
Date
2011
Source
PLoS One   6(5): e19713 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Animals
  • Methods
  • Immunohistochemistry/methods*
  • Embryo, Nonmammalian/chemistry*
  • Proteins/analysis
  • Fishes
  • Antigens/analysis*
(all 7)
PubMed
21603650 Full text @ PLoS One
Abstract

BACKGROUND:

For the detection and sub-cellular (co)-localization of proteins in the context of the tissue or organism immunostaining in whole mount preparations or on sections is still the best approach. So far, each antibody required its own fixation and antigen retrieval protocol so that optimizing immunostaining turned out to be tedious and time consuming.

METHODOLOGY/PRINCIPAL FINDING:

Here we present a novel method to efficiently retrieve the antigen in a widely applicable standard protocol, facilitating fluorescent immunostaining of both cryosections and whole mount preparations in zebrafish (Danio rerio) and medaka (Oryzias latipes).

CONCLUSIONS/SIGNIFICANCE:

Our method overcomes the loss of sections and damage of tissue and cell morphology, and allows parallel immunostaining in multiple colors, co-immunostaining with fluorescent proteins in transgenic fish lines and in combination with whole mount in situ hybridization.

Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cu2TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    cu2Tg
    1 - 1 of 1
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    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    RFPEFGRFP
    1 - 1 of 1
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    Mapping
    No data available
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    Citation
    Inoue, D., and Wittbrodt, J. (2011) One for all-a highly efficient and versatile method for fluorescent immunostaining in fish embryos. PLoS One. 6(5):e19713.