PUBLICATION

Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants

Authors
Saito, K., Siegfried, K.R., Nüsslein-Volhard, C., and Sakai, N.
ID
ZDB-PUB-110602-17
Date
2011
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   240(7): 1779-92 (Journal)
Registered Authors
Nüsslein-Volhard, Christiane, Sakai, Noriyoshi, Siegfried, Kellee
Keywords
male infertility, meiotic mutant, zebrafish, testis, spermatogenesis
MeSH Terms
  • Meiotic Prophase I/genetics*
  • Animals
  • Spermatogenesis/genetics
  • Spermatogenesis/physiology
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Testis/metabolism
  • Testis/physiology
  • Male
  • Infertility, Male/genetics
(all 10)
PubMed
21594953 Full text @ Dev. Dyn.
Abstract
We describe here the isolation and cytogenetic characterization of three meiotic prophase I mutants, denoted ietsugu (its), iesada (isa), and iemochi (imo), isolated by a novel N-ethyl-N-nitrosourea mutagenesis screen for adult zebrafish gonadogenesis. Histological examination and flow cytometry analysis of testes from these mutants showed that each contained neither spermatids nor sperm. Staining for Sycp3 and cleaved Caspase-3 and TUNEL (terminal deoxynucleotidyl transferase–mediated deoxyuridinetriphosphate nick end-labeling) assay further revealed that its had defects at the onset of meiosis, and that isa and imo spermatocytes failed to progress past the zygotene stage with apoptosis occurring in the testicular somatic cells. Staining for phosphorylated histone H2AX showed that foci formation in leptotene spermatocytes was disrupted in isa and imo. Furthermore, in vitro differentiation experiments revealed the possibility that the defects and sterility associated with mutations were germ line autonomous. Our results thus indicate that each responsible gene is necessary for meiotic progression during spermatogenesis and for male fertility.
Genes / Markers
Marker Marker Type Name
imoGENEiemochi
sycp1GENEsynaptonemal complex protein 1
sycp2GENEsynaptonemal complex protein 2
sycp3GENEsynaptonemal complex protein 3
zbtb16aGENEzinc finger and BTB domain containing 16a
1 - 5 of 5
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Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ha01TgTransgenic Insertion
    t30312
      Unknown
      t31556
        Point Mutation
        t32228
          Point Mutation
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-sycp3polyclonalRabbit
          Ab1-zbtb16apolyclonalRabbit
          1 - 2 of 2
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          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          1 - 1 of 1
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          Mapping
          No data available