PUBLICATION

Evx1 is required for joint formation in zebrafish fin dermoskeleton

Authors

Schulte, C.J., Allen, C., England, S.J., Juárez-Morales, J.L., and Lewis, K.E.

ID

ZDB-PUB-110518-28

Date

2011

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 240(5): 1240-1248 (Journal)

Registered Authors

Allen, Claire, England, Sam, Juárez-Morales, Jose-Luis, Lewis, Katharine E., Schulte, Claus

Keywords

segmentation, even-skipped, lepidotrichia, fins, zebrafish, evx

MeSH Terms

Animals
In Situ Hybridization
Gene Expression Regulation, Developmental
Zebrafish/embryology*
Zebrafish/metabolism*
Homeodomain Proteins/genetics
Homeodomain Proteins/metabolism*
Joints/embryology*
Joints/metabolism*
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Animal Fins/embryology
Animal Fins/metabolism*

(all 13)

PubMed

21509898 Full text @ Dev. Dyn.

Abstract

The transcription factor Evx1 is expressed in the joints between individual lepidotrichia (bony ray) segments and at the distal tips of the lepidotrichia in developing zebrafish fins. It is also expressed in the apical growth zone in regenerating fins. However, so far there is no functional evidence that addresses whether Evx1 is required for any aspect of fin development or regeneration. In this study, we use a novel mutation in evx1 to address this. We find that Evx1 is not required for either fin outgrowth or regeneration. All of the fins form normally in evx1 mutants, and there are no significant changes in fin length. In contrast, Evx1 is required for lepidotrichia joint formation during both fin development and regeneration. This is a very specific phenotype as both lepidotrichia hemisegment separations and lepidotrichia bifurcations still form normally in evx1 mutant fins, as do joints in the more proximal endoskeletal radials.

Genes / Markers

Marker	Marker Type	Name
evx1	GENE	even-skipped homeobox 1
gdf5	GENE	growth differentiation factor 5
gja5b	GENE	gap junction protein, alpha 5b
lof	GENE	long fin

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
evx1	AB	standard conditions	Days 30-44		anal fin lepidotrichium joint	ISH	Fig. 4
evx1	evx1^i232/i232 (AB)	standard conditions	Days 30-44	Not Detected	anal fin lepidotrichium joint	ISH	Fig. 4
gdf5	AB	standard conditions	Days 21-29	Not Detected	anal fin lepidotrichium joint	ISH	Fig. 4
gdf5	AB	standard conditions	Days 30-44	Not Detected	anal fin lepidotrichium joint	ISH	Fig. 4
gdf5	AB	standard conditions	Days 21-29		anal fin radial joint	ISH	Fig. 4
gdf5	AB	standard conditions	Days 30-44		anal fin radial joint	ISH	Fig. 4

1 - 6 of 6

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
evx1^i232/+ (AB)	standard conditions	Adult	caudal fin lepidotrichium morphology, normal	Fig. S1
evx1^i232/+ (TL)	standard conditions	Adult	pectoral fin lepidotrichium morphology, normal	Fig. S1
evx1^i232/i232 (AB)	standard conditions	Adult	caudal fin lepidotrichium joint absent, abnormal	Fig. S1
evx1^i232/i232 (AB)	standard conditions	Adult	caudal fin lepidotrichium undivided, abnormal	Fig. S1
evx1^i232/i232 (AB)	standard conditions	Adult	fin length, normal	Fig. S1
evx1^i232/i232 (AB)	standard conditions	Adult	fin morphogenesis process quality, abnormal	Fig. S1
evx1^i232/i232 (TL)	standard conditions	Days 21-29	fin morphogenesis process quality, abnormal	Fig. 1
evx1^i232/i232 (TL)	standard conditions	Days 21-29	lepidotrichium joint absent, abnormal	Fig. 1
evx1^i232/i232 (TL)	standard conditions	Days 21-29	lepidotrichium undivided, abnormal	Fig. 1
evx1^i232/i232 (TL)	standard conditions	Adult	fin morphogenesis process quality, abnormal	Fig. S1

1 - 10 of 15

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
dt2	Unknown	lof
i232	Point Mutation	evx1
t1	Point Mutation	gja5b

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
AB
evx1^i232/i232 (AB)
evx1^i232/i232 (TL)
evx1^i232/+ (AB)
evx1^i232/+ (TL)
gja5b^t1/t1
lof^dt2

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Schulte et al., 2011 ZDB-PUB-110518-28 PMID:21509898

Evx1 is required for joint formation in zebrafish fin dermoskeleton

Schulte et al., 2011

ZDB-PUB-110518-28
PMID:21509898