PUBLICATION

Dynamic analysis of BMP-responsive smad activity in live zebrafish embryos

Authors
Laux, D.W., Febbo, J.A., and Roman, B.L.
ID
ZDB-PUB-110316-14
Date
2011
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   240(3): 682-694 (Journal)
Registered Authors
Roman, Beth
Keywords
zebrafish, bone morphogenetic proteins, Smads, transgenic reporter
MeSH Terms
  • Immunohistochemistry
  • Bone Morphogenetic Protein Receptors, Type I/genetics
  • Bone Morphogenetic Protein Receptors, Type I/metabolism
  • Smad1 Protein/genetics
  • Smad1 Protein/metabolism
  • In Situ Hybridization
  • Smad5 Protein/genetics
  • Smad5 Protein/metabolism
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism*
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Animals
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Smad Proteins/genetics
  • Smad Proteins/metabolism*
  • Smad8 Protein/genetics
  • Smad8 Protein/metabolism
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 24)
PubMed
21337466 Full text @ Dev. Dyn.
Abstract
Bone morphogenetic proteins (BMPs) are critical players in development and disease, regulating such diverse processes as dorsoventral patterning, palate formation, and ossification. These ligands are classically considered to signal via BMP receptor-specific Smad proteins 1, 5, and 8. To determine the spatiotemporal pattern of Smad1/5/8 activity and thus canonical BMP signaling in the developing zebrafish embryo, we generated a transgenic line expressing EGFP under the control of a BMP-responsive element. EGFP is expressed in many established BMP signaling domains and is responsive to alterations in BMP type I receptor activity and smad1 and smad5 expression. This transgenic Smad1/5/8 reporter line will be useful for determining ligand and receptor requirements for specific domains of BMP activity, as well as for genetic and pharmacological screens aimed at identifying enhancers or suppressors of canonical BMP signaling.
Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
DsRed2f2Tg; pt510Tgstandard conditionsLong-pecIFL
DsRedxum13Tgstandard conditionsPrim-5IFL
DsRedxum13Tgstandard conditionsLong-pecIFL
DsRedxum13Tgstandard conditionsDay 4IFL
DsRedxum13Tgstandard conditionsPrim-5IFL
EGFPf2Tg; pt510Tgstandard conditionsLong-pecIFL
EGFPpt509Tgstandard conditionsPrim-5IFL
EGFPpt509Tgstandard conditionsPrim-5IFL
EGFPpt509Tgstandard conditionsPrim-5IFL
EGFPpt509Tgstandard conditionsPrim-5IFL
1 - 10 of 80
Show
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-smad1standard conditionsPrim-5
WT + MO1-smad1standard conditionsPrim-5
WT + MO1-smad1standard conditionsPrim-5
WT + MO1-smad1standard conditionsPrim-5
WT + MO1-smad1standard conditionsPrim-5
WT + MO1-smad1standard conditionsPrim-5
WT + MO1-smad1standard conditionsLong-pec
WT + MO1-smad1standard conditionsLong-pec
WT + MO1-smad1standard conditionsLong-pec
WT + MO1-smad1standard conditionsLong-pec
1 - 10 of 20
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
f2TgTransgenic Insertion
    pt509TgTransgenic Insertion
      pt510TgTransgenic Insertion
        um13TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          smad1MO1-smad1MRPHLNO
          smad5MO3-smad5MRPHLNO
          1 - 2 of 2
          Show
          Fish
          Fish
          f2Tg
          f2Tg; pt510Tg
          pt509Tg
          pt510Tg
          pt510Tg + MO1-smad1
          pt510Tg + MO3-smad5
          um13Tg
          WT + MO1-smad1
          WT + MO3-smad5
          1 - 9 of 9
          Show
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-smadpolyclonalIgGRabbit
          1 - 1 of 1
          Show
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          DsRed2EFGDsRed2
          DsRedxEFGDsRedx
          EGFPEFGEGFP
          1 - 3 of 3
          Show
          Mapping
          No data available
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          Citation
          Laux, D.W., Febbo, J.A., and Roman, B.L. (2011) Dynamic analysis of BMP-responsive smad activity in live zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(3):682-694.