PUBLICATION
Dynamic analysis of BMP-responsive smad activity in live zebrafish embryos
- Authors
- Laux, D.W., Febbo, J.A., and Roman, B.L.
- ID
- ZDB-PUB-110316-14
- Date
- 2011
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 240(3): 682-694 (Journal)
- Registered Authors
- Roman, Beth
- Keywords
- zebrafish, bone morphogenetic proteins, Smads, transgenic reporter
- MeSH Terms
-
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism*
- Immunohistochemistry
- Bone Morphogenetic Protein Receptors, Type I/genetics
- Bone Morphogenetic Protein Receptors, Type I/metabolism
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- In Situ Hybridization
- Bone Morphogenetic Proteins/genetics
- Bone Morphogenetic Proteins/metabolism*
- Smad5 Protein/genetics
- Smad5 Protein/metabolism
- Smad1 Protein/genetics
- Smad1 Protein/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Animals
- Smad Proteins/genetics
- Smad Proteins/metabolism*
- Smad8 Protein/genetics
- Smad8 Protein/metabolism
- PubMed
- 21337466 Full text @ Dev. Dyn.
Citation
Laux, D.W., Febbo, J.A., and Roman, B.L. (2011) Dynamic analysis of BMP-responsive smad activity in live zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(3):682-694.
Abstract
Bone morphogenetic proteins (BMPs) are critical players in development and disease, regulating such diverse processes as dorsoventral patterning, palate formation, and ossification. These ligands are classically considered to signal via BMP receptor-specific Smad proteins 1, 5, and 8. To determine the spatiotemporal pattern of Smad1/5/8 activity and thus canonical BMP signaling in the developing zebrafish embryo, we generated a transgenic line expressing EGFP under the control of a BMP-responsive element. EGFP is expressed in many established BMP signaling domains and is responsive to alterations in BMP type I receptor activity and smad1 and smad5 expression. This transgenic Smad1/5/8 reporter line will be useful for determining ligand and receptor requirements for specific domains of BMP activity, as well as for genetic and pharmacological screens aimed at identifying enhancers or suppressors of canonical BMP signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping