PUBLICATION

The gata1/pu.1 lineage fate paradigm varies between blood populations and is modulated by tif1γ

Authors
Monteiro, R., Pouget, C., and Patient, R.
ID
ZDB-PUB-110316-10
Date
2011
Source
The EMBO journal   30(6): 1093-1103 (Journal)
Registered Authors
Monteiro, Rui, Patient, Roger K.
Keywords
gata1, haematopoiesis, lineage fate decisions, pu.1, tif1γ
MeSH Terms
  • Gene Expression Regulation, Developmental*
  • Nuclear Proteins/metabolism*
  • GATA1 Transcription Factor/metabolism*
  • Embryo, Nonmammalian
  • Hematopoietic Stem Cells/physiology
  • Trans-Activators/metabolism*
  • Proto-Oncogene Proteins/metabolism*
  • Zebrafish*
  • Animals
  • Cell Differentiation*
  • Zebrafish Proteins/metabolism*
  • Transcription Factors/metabolism*
(all 12)
PubMed
21336259 Full text @ EMBO J.
Abstract
Lineage fate decisions underpin much of development as well as tissue homeostasis in the adult. A mechanistic paradigm for such decisions is the erythroid versus myeloid fate decision controlled by cross-antagonism between gata1 and pu.1 transcription factors. In this study, we have systematically tested this paradigm in blood-producing populations in zebrafish embryos, including the haematopoietic stem cells (HSCs), and found that it takes a different form in each population. In particular, gata1 activity varies from autostimulation to autorepression. In addition, we have added a third member to this regulatory kernel, tif1γ (transcription intermediate factor-1γ). We show that tif1γ modulates the erythroid versus myeloid fate outcomes from HSCs by differentially controlling the levels of gata1 and pu.1. By contrast, tif1γ positively regulates both gata1 and pu.1 in primitive erythroid and prodefinitive erythromyeloid progenitors. We therefore conclude that the gata1/pu.1 paradigm for lineage decisions takes different forms in different cellular contexts and is modulated by tif1γ.
Genes / Markers
Figures
No images available
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
la2TgTransgenic Insertion
    la781TgTransgenic Insertion
      tg234
        		Point Mutation
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        gata1aMO1-gata1aMRPHLNO
        gata1aMO2-gata1aMRPHLNO
        runx1MO1-runx1MRPHLNO
        spi1bMO1-spi1bMRPHLNO
        trim33MO3-trim33MRPHLNO
        1 - 5 of 5
        Show
        Fish
        Fish
        la781Tg
        trim33tg234/tg234; la2Tg
        1 - 2 of 2
        Show
        Antibodies
        Name Type Antigen Genes Isotypes Host Organism
        Ab1-casp3monoclonal
          		IgGRabbit
          1 - 1 of 1
          Show
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          GFPEFGGFP
          1 - 2 of 2
          Show
          Mapping
          No data available
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          Citation
          Monteiro, R., Pouget, C., and Patient, R. (2011) The gata1/pu.1 lineage fate paradigm varies between blood populations and is modulated by tif1γ. The EMBO journal. 30(6):1093-1103.