PUBLICATION

Brother of cdo (umleitung) is cell-autonomously required for Hedgehog-mediated ventral CNS patterning in the zebrafish

Authors
Bergeron, S.A., Tyurina, O.V., Miller, E., Bagas, A., and Karlstrom, R.O.
ID
ZDB-PUB-101201-47
Date
2011
Source
Development (Cambridge, England)   138(1): 75-85 (Journal)
Registered Authors
Bergeron, Sadie, Karlstrom, Rolf, Tyurina, Oksana
Keywords
Boc, Cdo, Shh, Morphogen, Pituitary, Jaw, Zebrafish
MeSH Terms
  • Prosencephalon/embryology
  • Signal Transduction/genetics
  • Signal Transduction/physiology
  • Animals
  • Immunohistochemistry
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Genotype
  • Axons/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • In Situ Hybridization
  • Codon, Nonsense/genetics
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/metabolism*
  • Central Nervous System/embryology*
  • Pituitary Gland/embryology
(all 17)
PubMed
21115611 Full text @ Development
Abstract
The transmembrane protein Brother of Cdo (Boc) has been implicated in Shh-mediated commissural axon guidance, and can both positively and negatively regulate Hedgehog (Hh) target gene transcription, however, little is known about in vivo requirements for Boc during vertebrate embryogenesis. The zebrafish umleitung (uml(ty54)) mutant was identified by defects in retinotectal axon projections. Here, we show that the uml locus encodes Boc and that Boc function is cell-autonomously required for Hh-mediated neural patterning. Our phenotypic analysis suggests that Boc is required as a positive regulator of Hh signaling in the spinal cord, hypothalamus, pituitary, somites and upper jaw, but that Boc might negatively regulate Hh signals in the lower jaw. This study reveals a role for Boc in ventral CNS cells that receive high levels of Hh and uncovers previously unknown roles for Boc in vertebrate embryogenesis.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
smo_unspecified
    Unspecified
    ts269
      Point Mutation
      ty54z
        Point Mutation
        ty119
          Point Mutation
          vu17TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            bocMO1-bocMRPHLNO
            1 - 1 of 1
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            Fish
            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            1 - 1 of 1
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            Mapping
            Entity Type Entity Symbol Location
            Featurety54zChr: 24 Details
            GENEbocChr: 24 Details
            GENEdrd3Chr: 24 Details
            SSLPz9852Chr: 24 Details
            1 - 4 of 4
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