PUBLICATION

Glial cell line-derived neurotrophic factor defines the path of developing and regenerating axons in the lateral line system of zebrafish

Authors
Schuster, K., Dambly-Chaudière, C., and Ghysen, A.
ID
ZDB-PUB-101027-47
Date
2010
Source
Proceedings of the National Academy of Sciences of the United States of America   107(45): 19531-19536 (Journal)
Registered Authors
Dambly-Chaudière, Christine, Ghysen, Alain
Keywords
axonal guidance, nerve regeneration, posterior lateral line, RET
MeSH Terms
  • Animals
  • Synaptic Transmission
  • Zebrafish
  • Lateral Line System/physiology*
  • Nerve Regeneration/physiology*
  • Sensory Receptor Cells
  • Glial Cell Line-Derived Neurotrophic Factor/physiology*
  • Axons/physiology*
  • Signal Transduction/physiology
  • Neurites
(all 10)
PubMed
20974953 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
How the peripheral axons of sensory neurons are guided to distant target organs is not well understood. Here we examine this question in the case of the posterior lateral line (PLL) system of zebrafish, where sensory organs are deposited by a migrating primordium. Sensory neurites accompany this primordium during its migration and are thereby guided to their prospective target organs. We show that the inactivation of glial cell line-derived neurotrophic factor (GDNF) signaling leads to defects of innervation and that these defects are due to the inability of sensory axons to track the migrating primordium. GDNF signaling is also used as a guidance cue during axonal regeneration following nerve cut. We conclude that GDNF is a major determinant of directed neuritic growth and of target finding in this system, and we propose that GDNF acts by promoting local neurite outgrowth.
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
rw0130aTgTransgenic Insertion
    sqet20EtTransgenic Insertion
      ump1TgTransgenic Insertion
        zf15TgTransgenic Insertion
          zf106TgTransgenic Insertion
            zf148TgTransgenic Insertion
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              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              gdnfaMO1-gdnfaMRPHLNO
              gdnfaMO2-gdnfaMRPHLNO
              gfra1aMO1-gfra1aMRPHLNO
              gfra1bMO1-gfra1bMRPHLNO
              retMO1-retMRPHLNO
              retMO2-retMRPHLNO
              1 - 6 of 6
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              Engineered Foreign Genes
              Marker Marker Type Name
              DsRedEFGDsRed
              EGFPEFGEGFP
              GFPEFGGFP
              KaedeEFGKaede
              mRFPEFGmRFP
              1 - 5 of 5
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