PUBLICATION

Characterization of zebrafish intestinal smooth muscle development using a novel sm22{alpha}-b promoter

Authors
Seiler, C., Abrams, J., and Pack, M.
ID
ZDB-PUB-101011-30
Date
2010
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   239(11): 2806-2812 (Journal)
Registered Authors
Pack, Michael, Seiler, Christoph
Keywords
zebrafish, smooth muscle, sm22a, transgelin, promoter, enteric nervous system ENS, transgenic, intestine, evolutionary conserved element (ECR), serum response element (SRE), colourless (cls)
MeSH Terms
  • Microfilament Proteins/genetics*
  • Promoter Regions, Genetic/genetics*
  • Enteric Nervous System/growth & development
  • Enteric Nervous System/metabolism
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • Zebrafish Proteins/genetics*
  • Zebrafish/embryology*
  • Animals
  • Intestines/embryology*
  • Muscle Proteins/genetics*
  • Muscle, Smooth/cytology
  • Muscle, Smooth/embryology*
  • Muscle, Smooth/growth & development
  • Muscle, Smooth/metabolism*
(all 15)
PubMed
20882680 Full text @ Dev. Dyn.
Abstract
Smooth muscle cells provide structural support for many tissues and control essential physiological processes, such as blood pressure and gastrointestinal motility. Relatively little is known about the early stages of intestinal smooth muscle development and its relationship to the development of the enteric nervous system, which regulates intestinal motility. Here, we report an evolutionarily conserved 523 base pair regulatory element within the promoter of the zebrafish sm22α-b (transgelin1) gene that directs transgene expression in smooth muscle cells of the intestine and other tissues. Comparative genomic analysis identified a conserved motif within this element consisting of two Serum Response Factor binding sites that is also present in the promoters of many mammalian smooth muscle genes. We established a stable line expressing GFP in smooth muscle cell and used this line to describe lineage relationships among cells within different intestinal smooth muscle layers and their co-development with the enteric nervous system (ENS).
Genes / Markers
Figures
Figure Gallery (4 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
p151TgTransgenic Insertion
    zf148TgTransgenic Insertion
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      Fish
      p151Tg
      p151Tg; zf148Tg
      1 - 2 of 2
      Show
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      DsRedEFGDsRed
      GFPEFGGFP
      1 - 2 of 2
      Show
      Mapping
      No data available
      Your Input Welcome
      We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
      Citation
      Seiler, C., Abrams, J., and Pack, M. (2010) Characterization of zebrafish intestinal smooth muscle development using a novel sm22{alpha}-b promoter. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(11):2806-2812.