PUBLICATION

Wnt11 patterns a myocardial electrical gradient through regulation of the L-type Ca(2+) channel

Authors
Panáková, D., Werdich, A.A., and MacRae, C.A.
ID
ZDB-PUB-100730-8
Date
2010
Source
Nature   466(7308): 874-878 (Journal)
Registered Authors
MacRae, Calum A., Panáková, Daniela
Keywords
none
MeSH Terms
  • Electric Conductivity*
  • Calcium Channels, L-Type/metabolism*
  • Myocytes, Cardiac/metabolism
  • Wnt Proteins/deficiency
  • Wnt Proteins/genetics
  • Wnt Proteins/metabolism*
  • Calcium Signaling
  • Signal Transduction*
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Myocardium/cytology
  • Myocardium/metabolism*
  • Calcium/metabolism
  • Heart/embryology
  • Animals
  • Ion Channel Gating/physiology*
(all 19)
PubMed
20657579 Full text @ Nature
Abstract
Electrical gradients are critical for many biological processes, including the normal function of excitable tissues, left-right patterning, organogenesis and wound healing. The fundamental mechanisms that regulate the establishment and maintenance of such electrical polarities are poorly understood. Here we identify a gradient of electrical coupling across the developing ventricular myocardium using high-speed optical mapping of transmembrane potentials and calcium concentrations in the zebrafish heart. We excluded a role for differences in cellular excitability, connexin localization, tissue geometry and mechanical inputs, but in contrast we were able to demonstrate that non-canonical Wnt11 signals are required for the genesis of this myocardial electrical gradient. Although the traditional planar cell polarity pathway is not involved, we obtained evidence that Wnt11 acts to set up this gradient of electrical coupling through effects on transmembrane Ca(2+) conductance mediated by the L-type calcium channel. These data reveal a previously unrecognized role for Wnt/Ca(2+) signalling in establishing an electrical gradient in the plane of the developing cardiac epithelium through modulation of ion-channel function. The regulation of cellular coupling through such mechanisms may be a general property of non-canonical Wnt signals.
Genes / Markers
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b109
    Small Deletion
    m209
      Point Mutation
      m231
        Unknown
        sb60
          Point Mutation
          tx226
            Point Mutation
            1 - 5 of 5
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            Human Disease / Model
            Sequence Targeting Reagents
            Fish
            Antibodies
            Orthology
            Engineered Foreign Genes
            No data available
            Mapping