PUBLICATION
Distinct roles for two synaptotagmin isoforms in synchronous and asynchronous transmitter release at zebrafish neuromuscular junction
- Authors
- Wen, H., Linhoff, M.W., McGinley, M.J., Li, G.L., Corson, G.M., Mandel, G., and Brehm, P.
- ID
- ZDB-PUB-100726-11
- Date
- 2010
- Source
- Proceedings of the National Academy of Sciences of the United States of America 107(31): 13906-13911 (Journal)
- Registered Authors
- Keywords
- active zone, exocytosis, synapse, acetylcholine receptor
- MeSH Terms
-
- Molecular Sequence Data
- Neuromuscular Junction/metabolism*
- Synaptotagmins/genetics
- Synaptotagmins/metabolism*
- Synaptic Transmission*
- PubMed
- 20643933 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
An obligatory role for the calcium sensor synaptotagmins in stimulus-coupled release of neurotransmitter is well established, but a role for synaptotagmin isoform involvement in asynchronous release remains conjecture. We show, at the zebrafish neuromuscular synapse, that two separate synaptotagmins underlie these processes. Specifically, knockdown of synaptotagmin 2 (syt2) reduces synchronous release, whereas knockdown of synaptotagmin 7 (syt7) reduces the asynchronous component of release. The zebrafish neuromuscular junction is unique in having a very small quantal content and a high release probability under conditions of either low-frequency stimulation or high-frequency augmentation. Through these features, we further determined that during the height of shared synchronous and asynchronous transmission these two modes compete for the same release sites.
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