PUBLICATION

Zebrafish Wnt9a,9b paralog comparisons suggest ancestral roles for Wnt9 in neural, oral-pharyngeal ectoderm and mesendoderm

Authors
Cox, A.A., Jezewski, P.A., Fang, P.K., and Payne-Ferreira, T.L.
ID
ZDB-PUB-100625-15
Date
2010
Source
Gene expression patterns : GEP   10(6): 251-258 (Journal)
Registered Authors
Jezewski, Peter, Payne-Ferreira, Tracie
Keywords
Zebrafish, Wnt signaling
MeSH Terms
  • Amino Acid Sequence
  • Ectoderm/embryology
  • Ectoderm/metabolism
  • Embryo, Nonmammalian
  • Wnt Proteins/genetics*
  • Wnt Proteins/physiology
  • Endoderm/embryology
  • Endoderm/metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Animals
  • Cloning, Molecular
  • Evolution, Molecular
  • Sequence Homology
  • Gene Expression Regulation, Developmental
  • Nervous System/embryology*
  • Nervous System/metabolism
  • Mouth/embryology*
  • Mouth/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/physiology
  • Branchial Region/embryology*
  • Branchial Region/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
(all 25)
PubMed
20566374 Full text @ Gene Expr. Patterns
Abstract
The Wnts are a highly conserved family of secreted glycoproteins involved in cell-cell signaling and pattern formation during early embryonic development. Teasing out the role of individual Wnt molecules through development is challenging. Gene duplications are one of the most important mechanisms for generating evolutionary variations. The current consensus suggests that most anatomical variation is generated by divergence of regulatory control regions rather than by coding sequence divergence. Thus phylogenetic comparisons of divergent gene expression patterns are essential to understanding ancestral morphogenetic patterns from which subsequent anatomy diversified in modern lineages. We previously demonstrated strongest expression of zebrafish wnt9b within its heart tube, limb bud and ventral/anterior ectoderm during oral and pharyngeal arch patterning. Our goal is to compare and contrast zwnt9b to its closest paralog, zwnt9a. Sequenced, fulllength zebrafish wnt9a and wnt9b cDNA clones were used for phylogenetic analysis, which suggests their derivation from a common pre-vertebrate archeolog by gene duplication and divergence. Here we demonstrate that zwnt9a expression is found within unique (CNS, pronephric ducts, sensory organs) and overlapping (pectoral fin buds) expression domains relative to zwnt9b. Apparently, Wnt9 paralogs differentially parsed common ancestral expression domains during their subsequent rounds of gene duplication, divergence and loss in different vertebrate lineages. This expression data suggests ancestral roles for Wnt9s in early patterning of neural/oral-pharyngeal ectoderm and mesendoderm derivatives.
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Citation
Cox, A.A., Jezewski, P.A., Fang, P.K., and Payne-Ferreira, T.L. (2010) Zebrafish Wnt9a,9b paralog comparisons suggest ancestral roles for Wnt9 in neural, oral-pharyngeal ectoderm and mesendoderm. Gene expression patterns : GEP. 10(6):251-258.