PUBLICATION

Identification of genes expressed by zebrafish oligodendrocytes using a differential microarray screen

Authors

Takada, N., and Appel, B.

ID

ZDB-PUB-100621-21

Date

2010

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 239(7): 2041-2047 (Journal)

Registered Authors

Appel, Bruce, Takada, Norio

Keywords

myelin, glia, differentiation, RNA profiling

MeSH Terms

Myelin Sheath/metabolism
Male
Gene Expression Regulation, Developmental*/genetics
Gene Expression Regulation, Developmental*/physiology
Oligodendroglia/cytology*
Oligodendroglia/metabolism*
Immunohistochemistry
Animals
Embryo, Nonmammalian/metabolism
Zebrafish/embryology*
Zebrafish/metabolism*
Oligonucleotide Array Sequence Analysis
Flow Cytometry
Female
In Situ Hybridization

(all 15)

PubMed

20549738 Full text @ Dev. Dyn.

Abstract

Myelination of central nervous system axons requires that oligodendrocytes extend multiple membrane processes that specifically recognize and wrap axons, which is followed by expression of proteins necessary for formation of myelin sheaths. To identify new genes that might be important for myelination, we used microarrays to analyze the expression profiles of cells sorted from transgenic zebrafish embryos and larvae under conditions that permitted or blocked oligodendrocyte development. Here, we describe eight genes that have not been previously implicated in oligodendrocyte development. Among the predicted functions of proteins encoded by these genes are lipid sensing, cell-cell junction formation, cytoskeleton regulation, and intracellular signaling. The predicted functions raise the possibility that these genes are involved in multiple cellular events during oligodendrocyte differentiation and myelin formation.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
cldnk	AB	control	Day 4		hindbrain oligodendrocyte	ISH	Fig. 2
cldnk	AB	control	Day 4		lateral line nerve myelinating Schwann cell	ISH	Fig. 3
cldnk	AB	control	Day 4		spinal cord oligodendrocyte	ISH	Fig. 3
cldnk	AB	control	Day 4		spinal cord oligodendrocyte	ISH	Fig. 2
mbpa	AB	control	Day 4		lateral line nerve myelinating Schwann cell	ISH	Fig. 3
mbpa	vu12Tg	control	Protruding-mouth		spinal cord oligodendrocyte	ISH	Fig. 1
mbpa	vu12Tg	chemical treatment: pharmaceutical	Protruding-mouth	Not Detected	spinal cord oligodendrocyte	ISH	Fig. 1
mid1ip1b	AB	control	Day 4		hindbrain oligodendrocyte	ISH	Fig. 2
mid1ip1b	AB	control	Day 4		lateral line nerve myelinating Schwann cell	ISH	Fig. 3
mid1ip1b	AB	control	Day 4		spinal cord oligodendrocyte	ISH	Fig. 2

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Phenotype

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
vu12Tg	Tg(olig2:EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
AB
vu12Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Takada et al., 2010 ZDB-PUB-100621-21 PMID:20549738

Identification of genes expressed by zebrafish oligodendrocytes using a differential microarray screen

Takada et al., 2010

ZDB-PUB-100621-21
PMID:20549738