PUBLICATION

BMP regulation of myogenesis in zebrafish

Authors
Patterson, S.E., Bird, N.C., and Devoto, S.H.
ID
ZDB-PUB-100223-13
Date
2010
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   239(3): 806-817 (Journal)
Registered Authors
Bird, Nathan C., Devoto, Stephen Henri
Keywords
myogenesis, BMP4, zebrafish, pax7, dermomyotome, myotome
MeSH Terms
  • Myogenic Regulatory Factors/metabolism
  • Animals
  • Zebrafish
  • Somites
  • Gene Expression Regulation, Developmental*
  • Models, Biological
  • Cell Differentiation
  • Zebrafish Proteins/metabolism
  • Cell Nucleus/metabolism
  • Muscles/metabolism
  • Paired Box Transcription Factors/metabolism
  • Bone Morphogenetic Protein 4/metabolism*
  • Signal Transduction
  • Muscle Development/genetics
  • PAX7 Transcription Factor/metabolism*
(all 15)
PubMed
20151472 Full text @ Dev. Dyn.
Abstract
In amniotes, BMP signaling from lateral plate and dorsal neural tube inhibits differentiation of muscle precursors in the dermomyotome. Here, we show that BMPs are expressed adjacent to the dermomyotome during and after segmentation in zebrafish. In addition, downstream BMP pathway members are expressed within the somite during dermomyotome development. We also show that zebrafish dermomyotome is responsive to BMP throughout its development. Ectopic overexpression of Bmp2b increases expression of the muscle precursor marker pax3, and changes the time course of myoD expression. At later stages, overexpression increases the number of Pax7+ myogenic precursors, and delays muscle differentiation, as indicated by decreased numbers of MEF2+ nuclei, decreased number of multi-nucleated muscle fibers, and an increased myotome angle. In addition, we show that while BMP overexpression is sufficient to delay myogenic differentiation, inhibition of BMP does not detectably affect this process, suggesting that other factors redundantly inhibit myogenic differentiation.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
w30TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    w30Tg
    1 - 1 of 1
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    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    GFPEFGGFP
    1 - 1 of 1
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    Mapping
    No data available
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    Citation
    Patterson, S.E., Bird, N.C., and Devoto, S.H. (2010) BMP regulation of myogenesis in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(3):806-817.