PUBLICATION

Hedgehog signaling and laminin play unique and synergistic roles in muscle development

Authors

Peterson, M.T., and Henry, C.A.

ID

ZDB-PUB-100112-30

Date

2010

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 239(3): 905-913 (Journal)

Registered Authors

Henry, Clarissa A.

Keywords

none

MeSH Terms

Muscle Fibers, Fast-Twitch/metabolism
Immunohistochemistry/methods
Developmental Biology/methods
Hedgehog Proteins/metabolism*
In Situ Hybridization
Animals
Signal Transduction
Muscle Development*
Myosins/metabolism
Muscles/embryology*
Zebrafish
Models, Biological
Gene Expression Regulation, Developmental*
Laminin/biosynthesis

(all 14)

PubMed

20063418 Full text @ Dev. Dyn.

Abstract

Hedgehog (Hh) signaling and laminin-111, a basement membrane protein, are required for early muscle development. Hh signaling specifies different populations of muscle fibers and laminin-111 is critical for early muscle morphogenesis. However, additional requirements for Hh signaling and laminin during later phases of muscle development are not known. Furthermore, interactions between Hh signaling and laminin in this context are unknown. We used laminin gamma1 mutant zebrafish and cyclopamine to block Hh signal transduction separately and in combination to investigate their functions and interactions. We found that both Hh signaling and laminin are required for normal myosin chain expression. In addition, Hh signaling and laminin act synergistically during fast-twitch fiber elongation: fast muscle cells do not elongate in embryos deficient for both Hh signaling and laminin. Finally, we present evidence that suggests that Hh signaling is indirectly required via slow fiber specification for recovery of fast fiber elongation in laminin gamma1 mutant embryos.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
myod1	lamc1^{ti263a/ti263a}	control	20-25 somites	somite	ISH	Fig. 1
myod1	lamc1^{ti263a/ti263a}	chemical treatment: Cyclopamine	20-25 somites	somite	ISH	Fig. 1
myod1	WT	chemical treatment: Cyclopamine	20-25 somites	somite	ISH	Fig. 1
myod1	WT	control	20-25 somites	somite	ISH	Fig. 1
myog	lamc1^{ti263a/ti263a}	chemical treatment: Cyclopamine	20-25 somites	somite	ISH	Fig. 1
myog	lamc1^{ti263a/ti263a}	control	20-25 somites	somite	ISH	Fig. 1
myog	WT	control	20-25 somites	somite	ISH	Fig. 1
myog	WT	chemical treatment: Cyclopamine	20-25 somites	somite	ISH	Fig. 1

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	chemical treatment: Cyclopamine	20-25 somites	muscle cell fate specification occurrence, normal	Fig. 1
WT	chemical treatment: Cyclopamine	20-25 somites	segmentation occurrence, normal	Fig. 1
WT	chemical treatment: Cyclopamine	20-25 somites	somite border present, normal	Fig. 1
WT	chemical treatment: Cyclopamine	20-25 somites	somite specification occurrence, normal	Fig. 1
WT	chemical treatment: Cyclopamine	Long-pec	fast muscle cell 2-D shape, normal	Fig. 2
WT	chemical treatment: Cyclopamine	Long-pec	fast muscle cell length, normal	Fig. 2
WT	chemical treatment: Cyclopamine	Long-pec	slow muscle cell decreased amount, abnormal	Fig. 3
WT + MO3-lamc1	chemical treatment: Cyclopamine	Long-pec	fast muscle cell circular, abnormal	Fig. 2
WT + MO3-lamc1	chemical treatment: Cyclopamine	Long-pec	fast muscle cell decreased length, abnormal	Fig. 2
WT + MO3-lamc1	standard conditions	Long-pec	fast muscle cell 2-D shape, normal	Fig. 2

1 - 10 of 27

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
ti263a		Unknown	lamc1

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
lamc1	MO3-lamc1	MRPHLNO

Fish

Fish
lamc1^{ti263a/ti263a}
WT
WT + MO3-lamc1

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Peterson et al., 2010 ZDB-PUB-100112-30 PMID:20063418

Hedgehog signaling and laminin play unique and synergistic roles in muscle development

Peterson et al., 2010

ZDB-PUB-100112-30
PMID:20063418