PUBLICATION

Chemokine signaling guides regional patterning of the first embryonic artery

Authors
Siekmann, A.F., Standley, C., Fogarty, K.E., Wolfe, S.A., and Lawson, N.D.
ID
ZDB-PUB-091023-4
Date
2009
Source
Genes & Development   23(19): 2272-2277 (Journal)
Registered Authors
Lawson, Nathan, Siekmann, Arndt Friedrich, Wolfe, Scot A.
Keywords
Aorta, zebrafish, zinc finger nuclease, differentiation, patterning, chemokine
MeSH Terms
  • Aorta/embryology*
  • Body Patterning/physiology*
  • Receptors, CXCR4/deficiency
  • Receptors, CXCR4/genetics
  • Receptors, CXCR4/metabolism
  • Chemokines/physiology*
  • Signal Transduction*
  • Gene Expression Regulation, Developmental
  • Zebrafish/embryology*
  • Animals
  • Endothelial Cells/cytology
(all 11)
PubMed
19797767 Full text @ Genes & Dev.
Abstract
The aorta traverses the body, yet little is known about how it is patterned in different anatomical locations. Here, we show that the aorta develops from genetically distinct endothelial cells originating from diverse locations within the embryo. Furthermore, chemokine (C-X-C motif) receptor 4a (cxcr4a) is restricted to endothelial cells derived from anterior mesoderm, and is required specifically for formation of the lateral aortae. Cxcl12b, a cxcr4a ligand, is expressed in endoderm underlying the lateral aortae, and loss of cxcl12b phenocopies cxcr4a deficiency. These studies reveal unexpected endothelial diversity within the aorta that is necessary to facilitate its regional patterning by local cues.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-cxcl12bstandard conditionsPrim-15
WT + MO1-cxcl12bstandard conditionsPrim-15
WT + MO3-sox32standard conditionsPrim-5
WT + MO3-sox32standard conditionsPrim-15
WT + MO3-sox32standard conditionsPrim-15
WT + MO3-sox32standard conditionsPrim-15
WT + MO3-sox32standard conditionsPrim-15
cxcr4aum20/um20standard conditions90%-epiboly
cxcr4aum20/um20standard conditionsPrim-5
cxcr4aum20/um20standard conditionsPrim-5
1 - 10 of 36
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b160
    		Indel
    la116TgTransgenic Insertion
      um20
        		Small Deletion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        cxcl12aMO7-cxcl12aMRPHLNO
        cxcl12bMO1-cxcl12bMRPHLNO
        cxcr4aMO1-cxcr4aMRPHLNO
        sox32MO3-sox32MRPHLNO
        1 - 4 of 4
        Show
        Fish
        Fish
        cxcr4aum20/um20
        cxcr4aum20/um20; la116Tg
        la116Tg
        la116Tg + MO1-cxcl12b
        la116Tg + MO1-cxcr4a
        la116Tg + MO3-sox32
        tbxtab160/b160
        WT
        WT + MO1-cxcl12b
        WT + MO3-sox32
        1 - 10 of 10
        Show
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        GFPEFGGFP
        1 - 1 of 1
        Show
        Mapping
        No data available
        Your Input Welcome
        We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
        Citation
        Siekmann, A.F., Standley, C., Fogarty, K.E., Wolfe, S.A., and Lawson, N.D. (2009) Chemokine signaling guides regional patterning of the first embryonic artery. Genes & Development. 23(19):2272-2277.