PUBLICATION

Fish-specific duplicated dmrt2b contributes to a divergent function through Hedgehog pathway and maintains left-right asymmetry establishment function

Authors

Liu, S., Li, Z., and Gui, J.F.

ID

ZDB-PUB-091005-5

Date

2009

Source

PLoS One 4(9): e7261 (Journal)

Registered Authors

Li, Zhi

Keywords

Embryos, Hedgehog signaling, Zebrafish, Somites, Muscle development, Duplicated genes, Messenger RNA, Muscle fibers

MeSH Terms

Hedgehog Proteins/metabolism*
Amino Acid Sequence
Signal Transduction
Neural Crest/metabolism
Transcription Factors/genetics
Transcription Factors/physiology*
Zebrafish
Body Patterning*
Zebrafish Proteins/genetics
Zebrafish Proteins/physiology*
Gene Expression Regulation
Molecular Sequence Data
Mesoderm/metabolism
Sequence Homology, Amino Acid
Animals
Gene Duplication
DNA-Binding Proteins/genetics
DNA-Binding Proteins/physiology*
Oligonucleotides, Antisense/metabolism

(all 19)

PubMed

19789708 Full text @ PLoS One

Abstract

Gene duplication is thought to provide raw material for functional divergence and innovation. Fish-specific dmrt2b has been identified as a duplicated gene of the dmrt2a/terra in fish genomes, but its function has remained unclear. Here we reveal that Dmrt2b knockdown zebrafish embryos display a downward tail curvature and have U-shaped somites. Then, we demonstrate that Dmrt2b contributes to a divergent function in somitogenesis through Hedgehog pathway, because Dmrt2b knockdown reduces target gene expression of Hedgehog signaling, and also impairs slow muscle development and neural tube patterning through Hedgehog signaling. Moreover, the Dmrt2b morphants display defects in heart and visceral organ asymmetry, and, some lateral-plate mesoderm (LPM) markers expressed in left side are randomized. Together, these data indicate that fish-specific duplicated dmrt2b contributes to a divergent function in somitogenesis through Hedgehog pathway and maintains the common function for left-right asymmetry establishment.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
dmrt2a	WT + MO1-dmrt2b + MO2-dmrt2b	standard conditions	Prim-5	somite	ISH	Fig. 3
dmrt2a	WT + MO1-dmrt2b + MO2-dmrt2b	standard conditions	Prim-5	somite	WB	Fig. 3
dmrt2b	WT	standard conditions	Prim-5	somite	ISH	Fig. 1
dmrt2b	WT	standard conditions	14-19 somites	somite	ISH	Fig. 1
dmrt2b	WT	standard conditions	Bud to Prim-25	whole organism	WB	Fig. 1
dmrt2b	WT	standard conditions	Shield to Long-pec	whole organism	RTPCR	Fig. 1
foxa3	WT	control	Long-pec	liver	ISH	Fig. 6
foxa3	WT + MO1-dmrt2b + MO2-dmrt2b	standard conditions	Long-pec	liver	ISH	Fig. 6
lft1	WT	control	20-25 somites	diencephalon	ISH	Fig. 7
lft1	WT	control	20-25 somites	lateral plate mesoderm	ISH	Fig. 7

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-dmrt2b	standard conditions	Prim-5	somite U-shaped, abnormal	Fig. 2
WT + MO1-dmrt2b	standard conditions	Prim-5	trunk curved ventral, abnormal	Fig. 2
WT + MO1-dmrt2b	standard conditions	Prim-5	trunk decreased length, abnormal	Fig. 2
WT + MO1-dmrt2b + MO2-dmrt2b	standard conditions	Prim-5	muscle pioneer decreased amount, abnormal	Fig. 4
WT + MO1-dmrt2b + MO2-dmrt2b	standard conditions	Prim-5	slow muscle cell decreased amount, abnormal	Fig. 4
WT + MO1-dmrt2b + MO2-dmrt2b	standard conditions	Prim-5	somite U-shaped, abnormal	Fig. 2
WT + MO1-dmrt2b + MO2-dmrt2b	standard conditions	Prim-5	trunk curved ventral, abnormal	Fig. 2
WT + MO1-dmrt2b + MO2-dmrt2b	standard conditions	Prim-5	trunk decreased length, abnormal	Fig. 2
WT + MO1-dmrt2b + MO2-dmrt2b	standard conditions	Long-pec	determination of left/right symmetry disrupted, abnormal	Fig. 6
WT + MO1-dmrt2b + MO2-dmrt2b	standard conditions	Long-pec	heart symmetry, abnormal	Fig. 6

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
dmrt2a	MO1-dmrt2a	MRPHLNO
dmrt2b	MO1-dmrt2b	MRPHLNO
dmrt2b	MO2-dmrt2b	MRPHLNO
sufu	MO3-sufu	MRPHLNO
tp53	MO4-tp53	MRPHLNO

Fish

Fish
WT
WT + MO1-dmrt2b
WT + MO1-dmrt2b + MO2-dmrt2b
WT + MO1-dmrt2b + MO2-dmrt2b + MO3-sufu
WT + MO2-dmrt2b
WT + MO3-sufu

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Liu et al., 2009 ZDB-PUB-091005-5 PMID:19789708

Fish-specific duplicated dmrt2b contributes to a divergent function through Hedgehog pathway and maintains left-right asymmetry establishment function

Liu et al., 2009

ZDB-PUB-091005-5
PMID:19789708