PUBLICATION

Fish-specific duplicated dmrt2b contributes to a divergent function through Hedgehog pathway and maintains left-right asymmetry establishment function

Authors
Liu, S., Li, Z., and Gui, J.F.
ID
ZDB-PUB-091005-5
Date
2009
Source
PLoS One   4(9): e7261 (Journal)
Registered Authors
Li, Zhi
Keywords
Embryos, Hedgehog signaling, Zebrafish, Somites, Muscle development, Duplicated genes, Messenger RNA, Muscle fibers
MeSH Terms
  • Hedgehog Proteins/metabolism*
  • Amino Acid Sequence
  • Signal Transduction
  • Neural Crest/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/physiology*
  • Zebrafish
  • Body Patterning*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Gene Expression Regulation
  • Molecular Sequence Data
  • Mesoderm/metabolism
  • Sequence Homology, Amino Acid
  • Animals
  • Gene Duplication
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/physiology*
  • Oligonucleotides, Antisense/metabolism
(all 19)
PubMed
19789708 Full text @ PLoS One
Abstract
Gene duplication is thought to provide raw material for functional divergence and innovation. Fish-specific dmrt2b has been identified as a duplicated gene of the dmrt2a/terra in fish genomes, but its function has remained unclear. Here we reveal that Dmrt2b knockdown zebrafish embryos display a downward tail curvature and have U-shaped somites. Then, we demonstrate that Dmrt2b contributes to a divergent function in somitogenesis through Hedgehog pathway, because Dmrt2b knockdown reduces target gene expression of Hedgehog signaling, and also impairs slow muscle development and neural tube patterning through Hedgehog signaling. Moreover, the Dmrt2b morphants display defects in heart and visceral organ asymmetry, and, some lateral-plate mesoderm (LPM) markers expressed in left side are randomized. Together, these data indicate that fish-specific duplicated dmrt2b contributes to a divergent function in somitogenesis through Hedgehog pathway and maintains the common function for left-right asymmetry establishment.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
dmrt2aMO1-dmrt2aMRPHLNO
dmrt2bMO1-dmrt2bMRPHLNO
dmrt2bMO2-dmrt2bMRPHLNO
sufuMO3-sufuMRPHLNO
tp53MO4-tp53MRPHLNO
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Fish
Antibodies
Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
No data available