PUBLICATION
Fish-specific duplicated dmrt2b contributes to a divergent function through Hedgehog pathway and maintains left-right asymmetry establishment function
- Authors
- Liu, S., Li, Z., and Gui, J.F.
- ID
- ZDB-PUB-091005-5
- Date
- 2009
- Source
- PLoS One 4(9): e7261 (Journal)
- Registered Authors
- Li, Zhi
- Keywords
- Embryos, Hedgehog signaling, Zebrafish, Somites, Muscle development, Duplicated genes, Messenger RNA, Muscle fibers
- MeSH Terms
-
- Mesoderm/metabolism
- Sequence Homology, Amino Acid
- Signal Transduction
- Body Patterning*
- Gene Duplication
- Neural Crest/metabolism
- Amino Acid Sequence
- Gene Expression Regulation
- Oligonucleotides, Antisense/metabolism
- Zebrafish
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/physiology*
- Hedgehog Proteins/metabolism*
- Molecular Sequence Data
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Transcription Factors/genetics
- Transcription Factors/physiology*
- Animals
- PubMed
- 19789708 Full text @ PLoS One
Citation
Liu, S., Li, Z., and Gui, J.F. (2009) Fish-specific duplicated dmrt2b contributes to a divergent function through Hedgehog pathway and maintains left-right asymmetry establishment function. PLoS One. 4(9):e7261.
Abstract
Gene duplication is thought to provide raw material for functional divergence and innovation. Fish-specific dmrt2b has been identified as a duplicated gene of the dmrt2a/terra in fish genomes, but its function has remained unclear. Here we reveal that Dmrt2b knockdown zebrafish embryos display a downward tail curvature and have U-shaped somites. Then, we demonstrate that Dmrt2b contributes to a divergent function in somitogenesis through Hedgehog pathway, because Dmrt2b knockdown reduces target gene expression of Hedgehog signaling, and also impairs slow muscle development and neural tube patterning through Hedgehog signaling. Moreover, the Dmrt2b morphants display defects in heart and visceral organ asymmetry, and, some lateral-plate mesoderm (LPM) markers expressed in left side are randomized. Together, these data indicate that fish-specific duplicated dmrt2b contributes to a divergent function in somitogenesis through Hedgehog pathway and maintains the common function for left-right asymmetry establishment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping