PUBLICATION

Protocadherin-19 is essential for early steps in brain morphogenesis

Authors

Emond, M.R., Biswas, S., and Jontes, J.D.

ID

ZDB-PUB-090727-4

Date

2009

Source

Developmental Biology 334(1): 72-83 (Journal)

Registered Authors

Emond, Michelle, Jontes, James

Keywords

Zebrafish, protocadherin, brain morphogenesis, neurulation, convergence

MeSH Terms

In Situ Hybridization
COS Cells
Body Patterning
Morphogenesis*
Cadherins/genetics
Cadherins/metabolism*
Chlorocebus aethiops
Nervous System/growth & development
Animals
Embryo, Nonmammalian/metabolism
Zebrafish
Neural Plate/growth & development*
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*

(all 14)

PubMed

19615992 Full text @ Dev. Biol.

Abstract

One of the earliest stages of brain morphogenesis is the establishment of the neural tube during neurulation. While some of the cellular mechanisms responsible for neurulation have been described in a number of vertebrate species, the underlying molecular processes are not fully understood. We have identified the zebrafish homolog of protocadherin-19, a member of the cadherin superfamily, which is expressed in the anterior neural plate and is required for brain morphogenesis. Interference with Protocadherin-19 function with antisense morpholino oligonucleotides leads to a severe disruption in early brain morphogenesis. Despite these pronounced effects on neurulation, axial patterning of the neural tube appears normal, as assessed by in situ hybridization for otx2, pax2.1 and krox20. Characterization of embryos early in development by in vivo 2-photon time-lapse microscopy reveals that the observed disruption of morphogenesis results from an arrest of cell convergence in the anterior neural plate. These results provide the first functional data for protocadherin-19, demonstrating an essential role in early brain development.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-pcdh19	standard conditions	5-9 somites	convergent extension disrupted, abnormal	Fig. 7
WT + MO1-pcdh19	standard conditions	5-9 somites	neural keel increased width, abnormal	Fig. 7
WT + MO1-pcdh19	standard conditions	5-9 somites to 10-13 somites	rostrocaudal neural tube patterning occurrence, normal	Fig. 8
WT + MO1-pcdh19	standard conditions	5-9 somites to 10-13 somites	somitogenesis process quality, normal	Fig. 8
WT + MO1-pcdh19	standard conditions	10-13 somites	brain disorganized, abnormal	Fig. 6
WT + MO1-pcdh19	standard conditions	10-13 somites	convergent extension disrupted, abnormal	Fig. 6
WT + MO1-pcdh19	standard conditions	10-13 somites	convergent extension disrupted, abnormal	Fig. 7
WT + MO1-pcdh19	standard conditions	10-13 somites	neural keel increased width, abnormal	Fig. 7
WT + MO1-pcdh19	standard conditions	10-13 somites	neural rod collapsed, abnormal	Fig. 6
WT + MO1-pcdh19	standard conditions	10-13 somites	neural rod disorganized, abnormal	Fig. 6

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
GFP	EFG	GFP

Mapping

Emond et al., 2009 ZDB-PUB-090727-4 PMID:19615992

Protocadherin-19 is essential for early steps in brain morphogenesis

Emond et al., 2009

ZDB-PUB-090727-4
PMID:19615992