PUBLICATION

Notch-responsive cells initiate the secondary transition in larval zebrafish pancreas

Authors
Parsons, M.J., Pisharath, H., Yusuff, S., Moore, J.C., Siekmann, A.F., Lawson, N., and Leach, S.D.
ID
ZDB-PUB-090716-21
Date
2009
Source
Mechanisms of Development   126(10): 898-912 (Journal)
Registered Authors
Lawson, Nathan, Leach, Steven D., Moore, John, Parsons, Michael, Siekmann, Arndt Friedrich
Keywords
Pancreatic progenitor, Endocrine differentiation, Zebrafish organogenesis, Secondary transition, Islet neogenesis
MeSH Terms
  • Receptors, Notch/physiology*
  • Fluorescent Dyes
  • Animals
  • Organogenesis
  • Microscopy, Electron, Transmission
  • Fluorescent Antibody Technique
  • Larva/growth & development*
  • Animals, Genetically Modified
  • Islets of Langerhans/cytology
  • Islets of Langerhans/embryology*
  • Islets of Langerhans/ultrastructure
  • Zebrafish/embryology*
(all 12)
PubMed
19595765 Full text @ Mech. Dev.
Abstract
Zebrafish provide a highly versatile model in which to study vertebrate development. Many recent studies have elucidated early events in the organogenesis of the zebrafish pancreas; however, several aspects of early endocrine pancreas formation in the zebrafish are not homologous to the mammalian system. To better identify mechanisms of islet formation in the zebrafish, with true homology to those observed in mammals, we have temporally and spatially characterized zebrafish secondary islet formation. As is the case in the mouse, we show that Notch inhibition leads to precocious differentiation of endocrine tissues. Furthermore, we have used transgenic fish expressing fluorescent markers under the control of a Notch-responsive element to observe the precursors of these induced endocrine cells. These pancreatic Notch-responsive cells represent a novel population of putative progenitors that are associated with larval pancreatic ductal epithelium, suggesting functional homology between secondary islet formation in zebrafish and the secondary transition in mammals. We also show that Notch-responsive cells persist in the adult pancreas and possess the classical characteristics of centroacinar cells, a cell type believed to be a multipotent progenitor cell in adult mammalian pancreas.
Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ia1TgTransgenic Insertion
    ia3TgTransgenic Insertion
      jh1TgTransgenic Insertion
        jh2TgTransgenic Insertion
          jh4TgTransgenic Insertion
            jh10TgTransgenic Insertion
              jh11TgTransgenic Insertion
                jh16TgTransgenic Insertion
                  jh17TgTransgenic Insertion
                    kca3TgTransgenic Insertion
                      1 - 10 of 14
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Fish
                      ia1Tg
                      ia3Tg
                      jh1Tg
                      jh2Tg
                      jh4Tg
                      jh10Tg
                      jh11Tg
                      jh16Tg
                      jh17Tg
                      kca3Tg
                      1 - 10 of 14
                      Show
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab2-inspolyclonalGuinea pig
                      1 - 1 of 1
                      Show
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GFPEFGGFP
                      grcfpEFGgrcfp
                      mCherryEFGmCherry
                      NTREFGNTR
                      1 - 6 of 6
                      Show
                      Mapping
                      No data available
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                      Citation
                      Parsons, M.J., Pisharath, H., Yusuff, S., Moore, J.C., Siekmann, A.F., Lawson, N., and Leach, S.D. (2009) Notch-responsive cells initiate the secondary transition in larval zebrafish pancreas. Mechanisms of Development. 126(10):898-912.