PUBLICATION

Apc1-Mediated Antagonism of Wnt/beta-Catenin Signaling Is Required for Retino-Tectal Pathfinding in the Zebrafish

Authors
Paridaen, J.T., Danesin, C., Elas, A.T., van de Water, S., Houart, C., and Zivkovic, D.
ID
ZDB-PUB-090319-17
Date
2009
Source
Zebrafish   6(1): 41-47 (Journal)
Registered Authors
Danesin, Cathy, Houart, Corinne
Keywords
none
MeSH Terms
  • Retina/cytology
  • Retina/embryology*
  • Retina/metabolism
  • Zebrafish/embryology*
  • Signal Transduction*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Retinal Ganglion Cells/metabolism
  • Tectum Mesencephali/cytology
  • Tectum Mesencephali/embryology*
  • Tectum Mesencephali/metabolism
  • Animals
  • beta Catenin/metabolism*
  • Embryo, Nonmammalian
  • Tumor Suppressor Proteins/genetics
  • Tumor Suppressor Proteins/metabolism*
  • Optic Nerve
  • Mutation
  • Axons
(all 19)
PubMed
19292673 Full text @ Zebrafish
Abstract
The tumor suppressor Apc1 is an intracellular antagonist of the Wnt/beta-catenin pathway. We examined the effects of an Apc1 loss-of-function mutation on retino-tectal axon pathfinding in zebrafish. In apc mutants, the retina is disorganized and optic nerves portray pathfinding defects at the optic chiasm and do not project properly to the tectum. Wild-type cells, transplanted into mutant retinae, acquire retinal ganglion cell fate and project axons that cross at the mispositioned optic chiasm and extend to the contralateral tectum, suggesting a function of apc1 in axon pathfinding. These defects are caused mainly by stabilization of beta-catenin. These data demonstrate that Apc1 function is required for correct patterning of the retina and proper retinal ganglion axon projections.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hu745
    Point Mutation
    w25TgTransgenic Insertion
      zf134
        Point Mutation
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        GFPEFGGFP
        1 - 1 of 1
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        Mapping
        No data available