PUBLICATION

Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryo

Authors
Schnapp, E., Pistocchi, A.S., Karampetsou, E., Foglia, E., Lamia, C.L., Cotelli, F., and Cossu, G.
ID
ZDB-PUB-090217-3
Date
2009
Source
Journal of Cell Science   122(Pt 4): 481-488 (Journal)
Registered Authors
Cotelli, Franco
Keywords
Skeletal myogenesis, MRF, Zebrafish, myf5, Myod, mrf4, Myogenin, Myotome
MeSH Terms
  • Animals
  • Muscle Development/physiology*
  • Myogenic Regulatory Factors/genetics
  • Myogenic Regulatory Factors/metabolism*
  • Zebrafish/embryology
  • Transcriptional Activation
  • Gene Deletion
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Myogenin/genetics
  • Myogenin/metabolism*
  • Myogenic Regulatory Factor 5/deficiency
  • Myogenic Regulatory Factor 5/genetics
  • MyoD Protein/genetics
  • MyoD Protein/metabolism
  • Gene Expression Regulation, Developmental/physiology
  • Time Factors
  • Gene Expression Profiling
  • Chromatin Assembly and Disassembly
(all 21)
PubMed
19193870 Full text @ J. Cell Sci.
Abstract
Muscle regulatory factors activate myogenesis in all vertebrates, but their role has been studied in great detail only in the mouse embryo, where all but myogenin - Myod, Myf5 and Mrf4 - are sufficient to activate (albeit not completely) skeletal myogenesis. In the zebrafish embryo, myod and myf5 are required for induction of myogenesis because their simultaneous ablation prevents muscle development. Here we show that mrf4 but not myog can fully rescue myogenesis in the myod/myf5 double morphant via a selective and robust activation of myod, in keeping with its chromatin-remodelling function in vitro. Rescue does not happen spontaneously, because the gene, unlike that in the mouse embryo, is expressed only at the onset of muscle differentiation, Moreover, because of the transient nature of morpholino inhibition, we were able to investigate how myogenesis occurs in the absence of a myotome. We report that in the complete absence of a myotome, subsequent myogenesis is abolished, whereas myogenesis does proceed, albeit abnormally, when the morpholino inhibition was not complete. Therefore our data also show that the early myotome is essential for subsequent skeletal muscle differentiation and patterning in the zebrafish.
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Citation
Schnapp, E., Pistocchi, A.S., Karampetsou, E., Foglia, E., Lamia, C.L., Cotelli, F., and Cossu, G. (2009) Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryo. Journal of Cell Science. 122(Pt 4):481-488.