PUBLICATION

Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryo

Authors

Schnapp, E., Pistocchi, A.S., Karampetsou, E., Foglia, E., Lamia, C.L., Cotelli, F., and Cossu, G.

ID

ZDB-PUB-090217-3

Date

2009

Source

Journal of Cell Science 122(Pt 4): 481-488 (Journal)

Registered Authors

Cotelli, Franco

Keywords

Skeletal myogenesis, MRF, Zebrafish, myf5, Myod, mrf4, Myogenin, Myotome

MeSH Terms

Transcriptional Activation
Gene Expression Profiling
Gene Deletion
Time Factors
Myogenic Regulatory Factors/genetics
Myogenic Regulatory Factors/metabolism*
Animals
Gene Expression Regulation, Developmental/physiology
Embryo, Nonmammalian/cytology
Embryo, Nonmammalian/physiology*
Myogenic Regulatory Factor 5/deficiency
Myogenic Regulatory Factor 5/genetics
MyoD Protein/genetics
MyoD Protein/metabolism
Muscle Development/physiology*
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Myogenin/genetics
Myogenin/metabolism*
Chromatin Assembly and Disassembly
Zebrafish/embryology

(all 21)

PubMed

19193870 Full text @ J. Cell Sci.

Abstract

Muscle regulatory factors activate myogenesis in all vertebrates, but their role has been studied in great detail only in the mouse embryo, where all but myogenin - Myod, Myf5 and Mrf4 - are sufficient to activate (albeit not completely) skeletal myogenesis. In the zebrafish embryo, myod and myf5 are required for induction of myogenesis because their simultaneous ablation prevents muscle development. Here we show that mrf4 but not myog can fully rescue myogenesis in the myod/myf5 double morphant via a selective and robust activation of myod, in keeping with its chromatin-remodelling function in vitro. Rescue does not happen spontaneously, because the gene, unlike that in the mouse embryo, is expressed only at the onset of muscle differentiation, Moreover, because of the transient nature of morpholino inhibition, we were able to investigate how myogenesis occurs in the absence of a myotome. We report that in the complete absence of a myotome, subsequent myogenesis is abolished, whereas myogenesis does proceed, albeit abnormally, when the morpholino inhibition was not complete. Therefore our data also show that the early myotome is essential for subsequent skeletal muscle differentiation and patterning in the zebrafish.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-myf5 + MO1-myod1	standard conditions	Prim-5	muscle apoptotic, abnormal	Fig. 5
AB + MO1-myf5 + MO1-myod1	standard conditions	Prim-5	muscle disorganized, abnormal	Fig. 5
AB + MO1-myf5 + MO1-myod1	standard conditions	Prim-5	muscle sarcomere disorganized, abnormal	Fig. 5
AB + MO1-myf5 + MO1-myod1	standard conditions	Prim-5	muscle sarcomere disoriented, abnormal	Fig. 5
AB + MO1-myf5 + MO1-myod1	standard conditions	Prim-5	whole organism movement quality, abnormal	Fig. 1

1 - 5 of 5

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
zf13Tg	Tg(actc1b:GFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
GFP	EFG	GFP

Mapping

Schnapp et al., 2009 ZDB-PUB-090217-3 PMID:19193870

Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryo

Schnapp et al., 2009

ZDB-PUB-090217-3
PMID:19193870