PUBLICATION

gfap and nestin reporter lines reveal characteristics of neural progenitors in the adult zebrafish brain

Authors
Lam, C.S., Maerz, M., and Strähle, U.
ID
ZDB-PUB-090204-2
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(2): 475-486 (Journal)
Registered Authors
Maerz, Martin, Strähle, Uwe
Keywords
adult neurogenesis, ventricular zone, telencephalon, transgenic line
MeSH Terms
  • Stem Cells/cytology*
  • Stem Cells/metabolism
  • Prosencephalon/cytology
  • Prosencephalon/metabolism
  • SOXB1 Transcription Factors/genetics
  • Intermediate Filament Proteins/genetics
  • Intermediate Filament Proteins/metabolism*
  • Neurons/cytology*
  • Neurons/metabolism
  • Nestin
  • Animals, Genetically Modified
  • Zebrafish/metabolism*
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism*
  • Glial Fibrillary Acidic Protein/genetics
  • Glial Fibrillary Acidic Protein/metabolism*
  • Genes, Reporter
  • Brain/cytology
  • Brain/metabolism*
  • Animals
(all 20)
PubMed
19161226 Full text @ Dev. Dyn.
Abstract
Adult neurogenesis arises from niches that harbor neural stem cells (NSC). Although holding great promise for regenerative medicine, the identity of NSC remains elusive. In mammals, a key attribute of NSC is the expression of the filamentous proteins glial fibrillary acidic protein (GFAP) and NESTIN. To assess whether these two markers are relevant in the fish model, two transgenic zebrafish lines for gfap and nestin were generated. Analysis of adult brains showed that the fusion GFAP-green fluorescent protein closely mimics endogenous GFAP, while the nestin transgene recapitulates nestin at the ventricular zones. Cells expressing the two reporters display radial glial morphology, colocalize with the NSC marker Sox2, undergo proliferation, and are capable of self-renewal within the matrix of distinct thickness in the telencephalon. Together, these two transgenic lines reveal a conserved feature of putative NSC in the adult zebrafish brain and provide a means for the identification and manipulation of these cells in vivo.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
gfapab1-gfapzf167Tgstandard conditionsAdultIHC
gfapab1-gfapzf167Tgstandard conditionsAdultIHC
gfapab1-gfapzf167Tgstandard conditionsAdultIHC
gfapab1-gfapzf167Tgstandard conditionsAdultIHC
gfapab1-gfapzf167Tgstandard conditionsAdultIHC
gfapab1-gfapzf167Tgstandard conditionsAdultIHC
gfapab1-gfapzf167Tgstandard conditionsAdultIHC
gfapab1-gfapzf167Tgstandard conditionsAdultIHC
GFPab3-gfpzf167Tgstandard conditionsAdultIHC
GFPab3-gfpzf167Tgstandard conditionsAdultIHC
1 - 10 of 28
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf167TgTransgenic Insertion
    zf168TgTransgenic Insertion
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      Fish
      zf167Tg
      zf168Tg
      1 - 2 of 2
      Show
      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab1-gfapmonoclonalIgG1Mouse
      Ab1-sox2polyclonalRabbit
      Ab2-pcnamonoclonalIgG2aMouse
      Ab3-GFPpolyclonalIgGRabbit
      1 - 4 of 4
      Show
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      1 - 1 of 1
      Show
      Mapping
      No data available
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      Citation
      Lam, C.S., Maerz, M., and Strähle, U. (2009) gfap and nestin reporter lines reveal characteristics of neural progenitors in the adult zebrafish brain. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(2):475-486.