PUBLICATION

Essential role of spi-1-like (spi-1l) in zebrafish myeloid cell differentiation

Authors

Bukrinsky, A., Griffin, K.J., Zhao, Y., Lin, S., and Banerjee, U.

ID

ZDB-PUB-090112-17

Date

2009

Source

Blood 113(9): 2038-2046 (Journal)

Registered Authors

Bukrinsky, Alex, Griffin, Kevin, Lin, Shuo, Zhao, Yan

Keywords

none

MeSH Terms

Embryo, Nonmammalian
Oligonucleotides, Antisense/pharmacology
Phylogeny
Morpholines/chemistry
Morpholines/pharmacology
Trans-Activators/chemistry
Trans-Activators/genetics
Trans-Activators/physiology
Sequence Homology, Amino Acid
Animals, Genetically Modified
Transcription Factors/chemistry
Transcription Factors/genetics
Transcription Factors/metabolism
Transcription Factors/physiology*
Proto-Oncogene Proteins/chemistry
Proto-Oncogene Proteins/genetics
Proto-Oncogene Proteins/physiology
Zebrafish/genetics
Zebrafish/physiology*
Amino Acid Sequence
Gene Expression Regulation, Developmental/drug effects
Cell Differentiation/genetics*
Zebrafish Proteins/chemistry
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Zebrafish Proteins/physiology*
Animals
Molecular Sequence Data
Protein Structure, Tertiary
Myeloid Cells/metabolism
Myeloid Cells/physiology*

(all 31)

PubMed

19131555 Full text @ Blood

Abstract

The ETS protein Spi-1/Pu.1 plays a pivotal and widespread role throughout hematopoiesis in many species. This study describes the identification, characterization and functional analysis of a new zebrafish spi transcription factor spi-1-like (spi-1l) that is expressed in primitive myeloid cells, erythro-myelo progenitor cells, and in the adult kidney. Spi-1l functions genetically downstream of etsrp, scl, and spi-1/pu.1 in myeloid differentiation. Spi-1l is co-expressed in a subset of spi-1/pu.1 cells and its function is necessary and sufficient for macrophage and granulocyte differentiation. These results establish a critical role for spi-1l in zebrafish myeloid cell differentiation.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
gata1a	WT	standard conditions	Prim-5		intermediate cell mass of mesoderm	ISH	Fig. 3
gata1a	WT + MO1-spi1b	standard conditions	Prim-5		anterior lateral plate mesoderm	ISH	Fig. 3
gata1a	WT + MO1-spi1b	standard conditions	Prim-5		intermediate cell mass of mesoderm	ISH	Fig. 3
gata1a	WT + MO2-spi1a	standard conditions	Prim-5		intermediate cell mass of mesoderm	ISH	Fig. 3
lcp1	Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39; s843Tg	standard conditions	Prim-25	Not Detected	yolk macrophage	ISH	Fig. 6
lcp1	s843Tg	standard conditions	Prim-25		yolk macrophage	ISH	Fig. 6
lcp1	WT	standard conditions	Prim-5		intermediate cell mass of mesoderm macrophage	ISH	Fig. 5
lcp1	WT	standard conditions	Prim-5		intermediate cell mass of mesoderm macrophage	ISH	Fig. 3
lcp1	WT	standard conditions	Prim-5		yolk macrophage	ISH	Fig. 3
lcp1	WT	standard conditions	Prim-5		yolk macrophage	ISH	Fig. 5

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39; s843Tg	standard conditions	Prim-25	blood island lacks parts or has fewer parts of type granulocyte, abnormal	Fig. 6
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39; s843Tg	standard conditions	Prim-25	blood island lacks parts or has fewer parts of type macrophage, abnormal	Fig. 6
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39; s843Tg	standard conditions	Prim-25	regulation of myeloid leukocyte differentiation disrupted, abnormal	Fig. 6
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39; s843Tg	standard conditions	Prim-25	yolk lacks parts or has fewer parts of type granulocyte, abnormal	Fig. 6
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39; s843Tg	standard conditions	Prim-25	yolk lacks parts or has fewer parts of type macrophage, abnormal	Fig. 6
WT + MO1-spi1b	standard conditions	Prim-5	blood island lacks parts or has fewer parts of type granulocyte, abnormal	Fig. 3
WT + MO1-spi1b	standard conditions	Prim-5	blood island lacks parts or has fewer parts of type granulocyte, abnormal	Fig. 5
WT + MO1-spi1b	standard conditions	Prim-5	blood island lacks parts or has fewer parts of type macrophage, abnormal	Fig. 3
WT + MO1-spi1b	standard conditions	Prim-5	blood island lacks parts or has fewer parts of type macrophage, abnormal	Fig. 5
WT + MO1-spi1b	standard conditions	Prim-5	regulation of myeloid leukocyte differentiation disrupted, abnormal	Fig. 4

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
m39		Deficiency	klhdc3 lclat1 npas4l slc30a10
s843Tg	Tg(kdrl:EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
etsrp	MO2-etsrp	MRPHLNO
spi1a	MO1-spi1a	MRPHLNO
spi1a	MO2-spi1a	MRPHLNO
spi1b	MO1-spi1b	MRPHLNO

Fish

Fish
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39; s843Tg
s843Tg
WT
WT + MO1-spi1b
WT + MO2-etsrp
WT + MO2-spi1a

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Bukrinsky et al., 2009 ZDB-PUB-090112-17 PMID:19131555

Essential role of spi-1-like (spi-1l) in zebrafish myeloid cell differentiation

Bukrinsky et al., 2009

ZDB-PUB-090112-17
PMID:19131555