PUBLICATION

Initiation and propagation of posterior to anterior (PA) waves in zebrafish left-right development

Authors

Wang, X., and Yost, H.J.

ID

ZDB-PUB-081110-6

Date

2008

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 237(12): 3640-3647 (Journal)

Registered Authors

Yost, H. Joseph

Keywords

left-right development, zebrafish, spaw, pitx2, lefty1, casanova, charon

MeSH Terms

Transcription Factors/genetics
Transcription Factors/metabolism
Embryo, Nonmammalian/embryology
Embryo, Nonmammalian/metabolism
Gene Expression Regulation, Developmental
Body Patterning*
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Animals, Genetically Modified
DNA-Binding Proteins/genetics
DNA-Binding Proteins/metabolism
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism
Animals
Homeodomain Proteins/genetics
Homeodomain Proteins/metabolism
Left-Right Determination Factors/genetics
Left-Right Determination Factors/metabolism

(all 19)

PubMed

18985756 Full text @ Dev. Dyn.

Abstract

One of the most highly conserved steps in left-right patterning is asymmetric gene expression in lateral plate mesoderm (LPM). Here, we quantitatively describe the timing of the posterior to anterior (PA) wave-like propagation of zebrafish southpaw (Nodal) and pitx2 in LPM and lefty1 in the midline. By altering the timing of the PA wave, we provide evidence that the PA wave in the LPM instructs brain asymmetry. We find that initiation of pitx2 in LPM and lefty1 in midline depends on Southpaw, and that casanova (sox32) and two Nodal inhibitors, lefty1 and charon, have distinct roles upstream of PA wave initiation. Surprisingly, Casanova, endoderm and Kupffer's Vesicle are not required for normal timing of southpaw initiation and PA propagation. In contrast, lefty1 morphants display precocious asymmetric initiation of southpaw with an intrinsic left-hand orientation, whereas charon morphants have premature initiation without LR orientation, indicating distinct roles for these Nodal antagonists.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
lft1	WT	standard conditions	10-13 somites		neural keel	ISH	Fig. 1
lft1	WT	standard conditions	14-19 somites		notochord	ISH	Fig. 2
lft1	WT	standard conditions	14-19 somites		notochord	ISH	Fig. 1
lft1	WT + MO1-spaw	standard conditions	14-19 somites	Not Detected	notochord	ISH	Fig. 2
lft1	WT + MO2-lft1	standard conditions	14-19 somites		brain	ISH	Fig. 5
lft1	WT + MO2-lft1	standard conditions	14-19 somites		notochord	ISH	Fig. 5
pitx2	WT	standard conditions	14-19 somites		lateral plate mesoderm	ISH	Fig. 1
pitx2	WT	standard conditions	14-19 somites		lateral plate mesoderm	ISH	Fig. 2
pitx2	WT	standard conditions	14-19 somites		Rohon-Beard neuron	ISH	Fig. 2
pitx2	WT + MO1-spaw	standard conditions	14-19 somites	Not Detected	lateral plate mesoderm	ISH	Fig. 2

1 - 10 of 25

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-dand5	standard conditions	10-13 somites	determination of left/right symmetry disrupted, abnormal	Fig. 3
WT + MO1-spaw	standard conditions	14-19 somites	lateral plate mesoderm pitx2 expression absent, abnormal	Fig. 2
WT + MO1-spaw	standard conditions	14-19 somites	notochord lft1 expression absent, abnormal	Fig. 2
WT + MO2-lft1	standard conditions	10-13 somites	determination of left/right symmetry disrupted, abnormal	Fig. 3
WT + MO2-sox32	standard conditions	10-13 somites	determination of left/right symmetry disrupted, abnormal	Fig. 3

1 - 5 of 5

Show

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
dand5	MO1-dand5	MRPHLNO
lft1	MO2-lft1	MRPHLNO
sox32	MO2-sox32	MRPHLNO
spaw	MO1-spaw	MRPHLNO

Fish

Fish
WT
WT + MO1-dand5
WT + MO1-spaw
WT + MO2-lft1
WT + MO2-sox32

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Wang et al., 2008 ZDB-PUB-081110-6 PMID:18985756

Initiation and propagation of posterior to anterior (PA) waves in zebrafish left-right development

Wang et al., 2008

ZDB-PUB-081110-6
PMID:18985756