PUBLICATION

Tailbud-derived Bmp4 drives proliferation and inhibits maturation of zebrafish chordamesoderm

Authors

Esterberg, R., Delalande, J.M., and Fritz, A.

ID

ZDB-PUB-081029-11

Date

2008

Source

Development (Cambridge, England) 135(23): 3891-3901 (Journal)

Registered Authors

Fritz, Andreas

Keywords

Bmp4, Chordamesoderm, Notochord, Zebrafish

MeSH Terms

Embryo, Nonmammalian/cytology
Embryo, Nonmammalian/drug effects
Embryo, Nonmammalian/metabolism
Animals
Bone Morphogenetic Protein 4/metabolism*
Ligands
Time Factors
Tail/embryology*
Mesoderm/cytology
Mesoderm/drug effects
Mesoderm/metabolism
Signal Transduction/drug effects
Gastrulation/drug effects
Zebrafish/embryology*
Oligonucleotides, Antisense/pharmacology
Cell Proliferation/drug effects
Notochord/cytology*
Notochord/drug effects
Notochord/embryology*
Body Patterning/drug effects

(all 20)

PubMed

18948415 Full text @ Development

Abstract

In zebrafish, BMP signaling establishes cell identity along the dorsoventral (DV) axis during gastrulation. Owing to the early requirements of BMP activity in DV patterning, it has been difficult to assign later roles in cell fate specification to specific BMP ligands. In this study, we have taken advantage of two follistatin-like genes (fstl1 and fstl2), as well as a transgenic zebrafish line carrying an inducible truncated form of the BMP-type 1 receptor to study the role of Bmp4 outside of the context of DV specification. Characterization of fstl1/2 suggests that they exert a redundant role as BMP antagonists during late gastrulation, regulating BMP activity in axial mesoderm. Maintenance of appropriate levels of BMP signaling is crucial for the proper development of chordamesoderm, a subset of axial mesoderm that gives rise to the notochord, but not prechordal mesoderm, which gives rise to the prechordal plate. Bmp4 activity in particular is required during a crucial window beginning at late gastrulation and lasting through early somitogenesis to promote chordamesoderm proliferation. In the absence of Bmp4, the notochord precursor pool is depleted, and the notochord differentiates prematurely. Our results illustrate a role for Bmp4 in the proliferation and timely differentiation of axial tissue after DV axis specification.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
admp	w30Tg	heat shock	90%-epiboly	axial chorda mesoderm	ISH	Fig. 2
admp	WT	control	90%-epiboly	axial chorda mesoderm	ISH	Fig. 2
admp	WT	control	75%-epiboly	axial chorda mesoderm	ISH	Fig. 2
admp	WT	control	5-9 somites	notochord posterior region	ISH	Fig. 1
admp	WT + MO2-fstl1a + MO3-bmp4 + MO4-fstl1b	standard conditions	90%-epiboly	axial chorda mesoderm	ISH	Fig. 2
admp	WT + MO2-fstl1a + MO4-fstl1b	standard conditions	75%-epiboly	axial chorda mesoderm	ISH	Fig. 2
admp	WT + MO2-fstl1a + MO4-fstl1b	standard conditions	90%-epiboly	axial chorda mesoderm	ISH	Fig. 2
admp	WT + MO2-fstl1a + MO4-fstl1b	standard conditions	5-9 somites	notochord posterior region	ISH	Fig. 1
admp	WT + MO3-bmp4	standard conditions	90%-epiboly	axial chorda mesoderm	ISH	Fig. 2
bmp4	WT	control	5-9 somites	tail bud	ISH	Fig. 1

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO2-fstl1a + MO3-bmp4 + MO4-fstl1b	standard conditions	90%-epiboly	axial chorda mesoderm decreased size, abnormal	Fig. 2
WT + MO2-fstl1a + MO4-fstl1b	standard conditions	90%-epiboly	axial chorda mesoderm increased size, abnormal	Fig. 2
WT + MO2-fstl1a + MO4-fstl1b	standard conditions	90%-epiboly	axial chorda mesoderm mislocalised anteriorly, abnormal	Fig. 2
WT + MO2-fstl1a + MO4-fstl1b	standard conditions	14-19 somites	chordo neural hinge increased size, abnormal	Fig. 3
WT + MO2-fstl1a + MO4-fstl1b	standard conditions	14-19 somites	notochord cell increased amount, abnormal	Fig. 3
WT + MO2-fstl1a + MO4-fstl1b	standard conditions	14-19 somites	notochord development disrupted, abnormal	Fig. 3
WT + MO2-fstl1a + MO4-fstl1b	standard conditions	14-19 somites	notochord development paedomorphic growth, abnormal	Fig. 5
WT + MO2-fstl1a + MO4-fstl1b	standard conditions	14-19 somites	notochord increased diameter, abnormal	Fig. 3
WT + MO2-fstl1a + MO4-fstl1b	standard conditions	Prim-5	chordo neural hinge increased size, abnormal	Fig. 3
WT + MO2-fstl1a + MO4-fstl1b	standard conditions	Prim-5	notochord cell decreased size, abnormal	Fig. 6

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
w30Tg	Tg(hsp70l:dnXla.Bmpr1a-GFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
bmp4	MO3-bmp4	MRPHLNO
fstl1a	MO1-fstl1a	MRPHLNO
fstl1a	MO2-fstl1a	MRPHLNO
fstl1b	MO3-fstl1b	MRPHLNO
fstl1b	MO4-fstl1b	MRPHLNO

Fish

Fish
w30Tg
WT
WT + MO2-fstl1a + MO3-bmp4 + MO4-fstl1b
WT + MO2-fstl1a + MO4-fstl1b
WT + MO3-bmp4

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-BrdU	monoclonal		IgG1	Mouse
Ab1-eng	monoclonal	en1a en1b en2a en2b	IgG1	Mouse
Ab1-myod1	polyclonal	myod1		Rabbit
Ab3-smad	polyclonal			Rabbit
Ab5-prox1	polyclonal	prox1a	IgG	Rabbit
Ab-F59	monoclonal		IgG1	Mouse
Ab-smad2	polyclonal	smad2		Rabbit

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Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
GFP	EFG	GFP

Mapping

Esterberg et al., 2008 ZDB-PUB-081029-11 PMID:18948415

Tailbud-derived Bmp4 drives proliferation and inhibits maturation of zebrafish chordamesoderm

Esterberg et al., 2008

ZDB-PUB-081029-11
PMID:18948415