PUBLICATION
Caprin-2 enhances canonical Wnt signaling through regulating LRP5/6 phosphorylation
- Authors
- Ding, Y., Xi, Y., Chen, T., Wang, J.Y., Tao, D.L., Wu, Z.L., Li, Y.P., Li, C., Zeng, R., and Li, L.
- ID
- ZDB-PUB-080908-2
- Date
- 2008
- Source
- The Journal of cell biology 182(5): 865-872 (Journal)
- Registered Authors
- Li, Lin
- Keywords
- none
- MeSH Terms
-
- Gene Silencing
- Repressor Proteins/metabolism
- Cytoplasm/metabolism
- Lymphoid Enhancer-Binding Factor 1/genetics
- Lymphoid Enhancer-Binding Factor 1/metabolism
- LDL-Receptor Related Proteins/metabolism*
- Wnt Proteins/genetics
- Wnt Proteins/metabolism*
- Low Density Lipoprotein Receptor-Related Protein-6
- Cell Line
- Glycogen Synthase Kinase 3/metabolism
- beta Catenin/metabolism
- Low Density Lipoprotein Receptor-Related Protein-5
- Zebrafish/embryology
- Zebrafish/metabolism
- Cell Cycle Proteins/genetics
- Cell Cycle Proteins/physiology*
- Axin Protein
- Humans
- Embryo, Nonmammalian/anatomy & histology
- Embryo, Nonmammalian/metabolism
- Phosphorylation
- TCF Transcription Factors/genetics
- TCF Transcription Factors/metabolism
- Phenotype
- Signal Transduction*
- Transcription, Genetic
- Animals
- Gene Expression Regulation
- PubMed
- 18762581 Full text @ J. Cell Biol.
Citation
Ding, Y., Xi, Y., Chen, T., Wang, J.Y., Tao, D.L., Wu, Z.L., Li, Y.P., Li, C., Zeng, R., and Li, L. (2008) Caprin-2 enhances canonical Wnt signaling through regulating LRP5/6 phosphorylation. The Journal of cell biology. 182(5):865-872.
Abstract
The low-density lipoprotein receptor-related proteins 5 and 6 (LRP5/6) are coreceptors for Frizzled and transmit signals from the plasma membrane to the cytosol. However, the mechanism for LRP5/6 signal transmission remains undefined. Here, we identify cytoplasmic activation/proliferation-associated protein 2 (Caprin-2) as a LRP5/6-binding protein. Our data show that Caprin-2 stabilizes cytosolic beta-catenin and enhances lymphoid enhancer-binding factor 1/T cell factor-dependent reporter gene activity as well as the expression of Wnt target genes in mammalian cells. Morpholino-mediated knockdown of Caprin-2 in zebrafish embryos inhibits Wnt/beta-catenin signaling and results in a dorsalized phenotype. Moreover, Caprin-2 facilitates LRP5/6 phosphorylation by glycogen synthase kinase 3, and thus enhances the interaction between Axin and LRP5/6. Therefore, Caprin-2 promotes activation of the canonical Wnt signaling pathway by regulating LRP5/6 phosphorylation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping