PUBLICATION

Zebrafish V2 cells develop into excitatory CiD and Notch signalling dependent inhibitory VeLD interneurons

Authors

Batista, M.F., Jacobstein, J., and Lewis, K.E.

ID

ZDB-PUB-080825-21

Date

2008

Source

Developmental Biology 322(2): 263-275 (Journal)

Registered Authors

Batista, Manuel, Jacobstein, Jeffrey, Lewis, Katharine E.

Keywords

V2, VeLD, CiD, KA, Notch, mindbomb, GABA, glycine, interneuron, spinal cord, chx10, vsx1, gata2, gata3, scl, zebrafish

MeSH Terms

Embryo, Nonmammalian/cytology
Embryo, Nonmammalian/metabolism
Spinal Cord/cytology
Spinal Cord/embryology*
Spinal Cord/metabolism
Antigens, Differentiation/metabolism
Animals
Interneurons/cytology
Interneurons/metabolism*
Zebrafish Proteins/metabolism*
Zebrafish/embryology*
Zebrafish/metabolism
Signal Transduction
Receptors, Notch/metabolism*
Cell Differentiation/physiology
Body Patterning
Motor Neurons/metabolism

(all 17)

PubMed

18680739 Full text @ Dev. Biol.

Abstract

The vertebrate spinal cord contains distinct classes of cells that form at precise dorsal-ventral locations and express specific combinations of transcription factors. In amniotes, V2 cells develop in the ventral spinal cord, just dorsal to motoneurons. All V2 cells develop from the same progenitor domain and hence are initially molecularly identical. However, as they start to become post-mitotic and differentiate they subdivide into two intermingled molecularly-distinct subpopulations of cells, V2a and V2b cells. Here we show that the molecular identities of V2a and V2b cells are conserved between zebrafish and amniotes. In zebrafish, these two cell types both develop into interneurons with very similar morphologies, but while V2a cells become excitatory Circumferential Descending (CiD) interneurons, V2b cells become inhibitory Ventral Lateral Descending (VeLD) interneurons. In addition, we demonstrate that Notch signalling is required for V2 cells to develop into V2b cells. In the absence of Notch signalling, all V2b cells develop as V2a cells.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
EGFP	zf100Tg	standard conditions	Prim-5		Kolmer-Agduhr neuron	IHC	Fig. 7
EGFP	zf100Tg	standard conditions	Prim-5		VeLD	IHC	Fig. 7
en1b	WT	standard conditions	Prim-5		spinal cord interneuron	ISH	Fig. 2
gad1b	mib1^ta52b/ta52b	standard conditions	Prim-5		spinal cord interneuron	ISH	Fig. 8
gad1b	WT	standard conditions	Prim-5	Not Detected	CiD	ISH	Fig. 5
gad1b	WT	standard conditions	Prim-5		VeLD	ISH	Fig. 5
gad1b	WT	standard conditions	Prim-5		VeLD	ISH	Fig. 8
gad1b	zf100Tg	standard conditions	Prim-5		Kolmer-Agduhr neuron	ISH	Fig. 7
gad2	mib1^ta52b/ta52b	standard conditions	Prim-5		spinal cord interneuron	ISH	Fig. 8
gad2	WT	standard conditions	Prim-5	Not Detected	CiD	ISH	Fig. 5

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
mib1^ta52b/ta52b	standard conditions	14-19 somites	CiD increased amount, abnormal	Fig. 8
mib1^ta52b/ta52b	standard conditions	14-19 somites	VeLD decreased amount, abnormal	Fig. 8
mib1^ta52b/ta52b	standard conditions	14-19 somites	ventral spinal cord interneuron differentiation disrupted, abnormal	Fig. 8
mib1^ta52b/ta52b	standard conditions	Prim-5	CiD increased amount, abnormal	Fig. 8
mib1^ta52b/ta52b	standard conditions	Prim-5	VeLD decreased amount, abnormal	Fig. 8
mib1^ta52b/ta52b	standard conditions	Prim-5	ventral spinal cord interneuron differentiation disrupted, abnormal	Fig. 8

1 - 6 of 6

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
nns2Tg	Tg(vsx2:Kaede)	Transgenic Insertion
ta52b		Point Mutation	mib1
zf100Tg	Tg(-8.1gata1:gata1-EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
mib1^ta52b/ta52b
nns2Tg
WT
zf100Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GFP	EFG	GFP
Kaede	EFG	Kaede

Mapping

Batista et al., 2008 ZDB-PUB-080825-21 PMID:18680739

Zebrafish V2 cells develop into excitatory CiD and Notch signalling dependent inhibitory VeLD interneurons

Batista et al., 2008

ZDB-PUB-080825-21
PMID:18680739