PUBLICATION

Origin and shaping of the laterality organ in zebrafish

Authors
Oteíza, P., Köppen, M., Concha, M.L., and Heisenberg, C.P.
ID
ZDB-PUB-080722-20
Date
2008
Source
Development (Cambridge, England)   135(16): 2807-2813 (Journal)
Registered Authors
Concha, Miguel, Heisenberg, Carl-Philipp
Keywords
Enveloping layer, Epiboly, Organizer, Organogenesis, Kupffer’s vesicle, Left-right asymmetry, Zebrafish
MeSH Terms
  • Cilia/physiology
  • Morphogenesis/physiology
  • Zebrafish Proteins/physiology*
  • Epithelium/embryology
  • Epithelium/physiology
  • Body Patterning/physiology
  • Cell Movement/physiology
  • Animals
  • Embryo, Nonmammalian/physiology
  • Cell Polarity/physiology
  • Transforming Growth Factor beta/physiology
  • Zebrafish/embryology*
  • Zebrafish/physiology
  • Nodal Protein/physiology
(all 14)
PubMed
18635607 Full text @ Development
Abstract
Handedness of the vertebrate body plan critically depends on transient embryonic structures/organs that generate cilia-dependent leftward fluid flow within constrained extracellular environments. Although the function of ciliated organs in laterality determination has been extensively studied, how they are formed during embryogenesis is still poorly understood. Here we show that Kupffer's vesicle (KV), the zebrafish organ of laterality, arises from a surface epithelium previously thought to adopt exclusively extra-embryonic fates. Live multi-photon confocal imaging reveals that surface epithelial cells undergo Nodal/TGFbeta signalling-dependent ingression at the dorsal germ ring margin prior to gastrulation, to give rise to dorsal forerunner cells (DFCs), the precursors of KV. DFCs then migrate attached to the overlying surface epithelium and rearrange into rosette-like epithelial structures at the end of gastrulation. During early somitogenesis, these epithelial rosettes coalesce into a single rosette that differentiates into the KV with a ciliated lumen at its apical centre. Our results provide novel insights into the morphogenetic transformations that shape the laterality organ in zebrafish and suggest a conserved progenitor role of the surface epithelium during laterality organ formation in vertebrates.
Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s870TgTransgenic Insertion
    vu119TgTransgenic Insertion
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      1 - 3 of 3
      Show
      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      GFPEFGGFP
      1 - 2 of 2
      Show
      Mapping
      No data available