PUBLICATION

Sox3 regulates both neural fate and differentiation in the zebrafish ectoderm

Authors
Dee, C.T., Hirst, C.S., Shih, Y.H., Tripathi, V.B., Patient, R.K., and Scotting, P.J.
ID
ZDB-PUB-080630-4
Date
2008
Source
Developmental Biology   320(1): 289-301 (Journal)
Registered Authors
Patient, Roger K., Shih, Yu-huan
Keywords
Sox3, Neural ectoderm, Neurogenesis, Zebrafish
MeSH Terms
  • Signal Transduction
  • High Mobility Group Proteins/genetics
  • High Mobility Group Proteins/metabolism*
  • Cell Differentiation*
  • 5' Untranslated Regions/genetics
  • Neural Plate/cytology
  • Neurons/cytology*
  • Neurons/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Molecular Sequence Data
  • Skull/abnormalities
  • Skull/embryology
  • Ear/abnormalities
  • Ear/embryology
  • Embryo, Nonmammalian/cytology
  • Fibroblast Growth Factors/metabolism
  • Cell Lineage*
  • Ectoderm/cytology*
  • Ectoderm/embryology
  • Gene Expression Regulation, Developmental
  • SOXB1 Transcription Factors
  • Animals
  • Base Sequence
  • Central Nervous System/embryology
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Biomarkers/metabolism
  • Body Patterning
(all 31)
PubMed
18572157 Full text @ Dev. Biol.
Abstract
Little is known of the first transcriptional events that regulate neural fate in response to extracellular signals such as Bmps and Fgfs. Sox3 is one of the earliest transcription factors to be expressed in the developing CNS and has been shown to be regulated by these signalling pathways. We have used both gain- and loss-of-function experiments in zebrafish to elucidate the role of Sox3 in determining neural fate. Ectopic Sox3 caused induction of neural tissue from a very early stage of cell specification in the ectoderm and this effect was maintained such that large domains of additional CNS were apparent, including almost complete duplications of the CNS. Knock-down of Sox3 using morpholinos resulted in a reduction in the size of the CNS, ears and eyes and subsequent inhibition of some aspects of neurogenesis. Our data also suggest that the pro-neural effects of Sox3 can compensate for inhibition of Fgf signalling in inducing neural tissue but it is not sufficient to maintain neural fate, suggesting the presence of Sox3-independent roles of Fgf at later stages.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
sox3MO1-sox3MRPHLNO
sox3MO2-sox3MRPHLNO
1 - 2 of 2
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Fish
1 - 2 of 2
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Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-Sox3polyclonalRabbit
Ab7-GFPpolyclonalIgGGoat
1 - 2 of 2
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Orthology
No data available
Engineered Foreign Genes
Marker Marker Type Name
GFPEFGGFP
1 - 1 of 1
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Mapping
No data available