PUBLICATION

Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation

Authors
Mizoguchi, T., Verkade, H., Heath, J.K., Kuroiwa, A., and Kikuchi, Y.
ID
ZDB-PUB-080630-12
Date
2008
Source
Development (Cambridge, England)   135(15): 2521-2529 (Journal)
Registered Authors
Heath, Joan K., Kikuchi, Yutaka, Mizoguchi, Takamasa, Verkade, Heather
Keywords
Zebrafish, Endoderm, Migration, Gastrulation, Sdf1 (Cxcl12), Cxcr4a
MeSH Terms
  • Cell Movement*
  • Chemotactic Factors/metabolism
  • Animals, Genetically Modified
  • Gene Expression Regulation, Developmental
  • Signal Transduction
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Gastrula/cytology
  • Gastrula/embryology*
  • Gastrula/metabolism*
  • Receptors, CXCR4/genetics
  • Receptors, CXCR4/metabolism*
  • Cell Shape
  • Chemokine CXCL12/genetics
  • Chemokine CXCL12/metabolism*
  • Endoderm/cytology
  • Endoderm/embryology*
  • Endoderm/metabolism
(all 20)
PubMed
18579679 Full text @ Development
Abstract
During vertebrate gastrulation, both mesodermal and endodermal cells internalize through the blastopore beneath the ectoderm. In zebrafish, the internalized mesodermal cells move towards the dorsal side of the gastrula and, at the same time, they extend anteriorly by convergence and extension (C&E) movements. Endodermal cells showing characteristic filopodia then migrate into the inner layer within the hypoblast next to the yolk syncytial layer (YSL). However, little is known about how the movement of endodermal cells is regulated during gastrulation. Here we show that sdf1a- and sdf1b-expressing mesodermal cells control the movements of the cxcr4a-expressing endodermal cells. The directional migration of endodermal cells during gastrulation is inhibited by knockdown of either cxcr4a or sdf1a/sdf1b (sdf1). We also show that misexpressed Sdf1 acts as a chemoattractant for cxcr4a-expressing endodermal cells. We further found, using the endoderm-specific transgenic line Tg(sox17:EGFP), that Sdf1/Cxcr4 signaling regulates both the formation and orientation of filopodial processes in endodermal cells. Moreover, the accumulation of phosphoinositide 3,4,5-trisphosphate (PIP3), which is known to occur at the leading edge of migrating cells, is not observed at the filopodia of endodermal cells. Based on our results, we propose that sdf1-expressing mesodermal cells, which overlie the endodermal layer, guide the cxcr4a-expressing endodermal cells to the dorsal side of the embryo during gastrulation, possibly through a PIP3-independent pathway.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ha01TgTransgenic Insertion
    s4
      Point Mutation
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      cxcl12aMO3-cxcl12aMRPHLNO
      cxcl12bMO1-cxcl12bMRPHLNO
      cxcr4aMO5-cxcr4aMRPHLNO
      cxcr4aMO6-cxcr4aMRPHLNO
      1 - 4 of 4
      Show
      Fish
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      1 - 1 of 1
      Show
      Mapping
      No data available