PUBLICATION

Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo

Authors

von Hofsten, J., Elworthy, S., Gilchrist, M.J., Smith, J.C., Wardle, F.C., and Ingham, P.W.

ID

ZDB-PUB-080616-5

Date

2008

Source

EMBO reports 9(7): 683-689 (Journal)

Registered Authors

Elworthy, Stone, Ingham, Philip, von Hofsten, Jonas, Wardle, Fiona

Keywords

none

Datasets

GEO:GSE10883

MeSH Terms

Cell Differentiation
Gene Expression Regulation, Developmental
Repressor Proteins/metabolism*
Organ Specificity
Chromatin Immunoprecipitation
Cardiac Myosins/genetics
DNA-Binding Proteins/metabolism*
Nuclear Proteins/metabolism*
Animals
Binding Sites
Muscle Fibers, Fast-Twitch/cytology
Transcription, Genetic*
Muscle Fibers, Skeletal/cytology
Muscle Fibers, Skeletal/metabolism*
Mutation/genetics
Up-Regulation/genetics
Myosin Light Chains/genetics
Promoter Regions, Genetic/genetics
Zebrafish Proteins/metabolism*
Zebrafish/embryology*
Zebrafish/genetics
Embryo, Nonmammalian/metabolism*
Muscle Fibers, Slow-Twitch/cytology

(all 23)

PubMed

18535625 Full text @ EMBO Rep.

Abstract

The zebrafish u-boot (ubo) gene encodes the transcription factor Prdm1, which is essential for the specification of the primary slow-twitch muscle fibres that derive from adaxial cells. Here, we show that Prdm1 functions by acting as a transcriptional repressor and that slow-twitch-specific muscle gene expression is activated by Prdm1-mediated repression of the transcriptional repressor Sox6. Genes encoding fast-specific isoforms of sarcomeric proteins are ectopically expressed in the adaxial cells of ubo(tp39) mutant embryos. By using chromatin immunoprecipitation, we show that these are direct targets of Prdm1. Thus, Prdm1 promotes slow-twitch fibre differentiation by acting as a global repressor of fast-fibre-specific genes, as well as by abrogating the repression of slow-fibre-specific genes.

Genes / Markers

Figures