PUBLICATION

Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo

Authors
von Hofsten, J., Elworthy, S., Gilchrist, M.J., Smith, J.C., Wardle, F.C., and Ingham, P.W.
ID
ZDB-PUB-080616-5
Date
2008
Source
EMBO reports   9(7): 683-689 (Journal)
Registered Authors
Elworthy, Stone, Ingham, Philip, von Hofsten, Jonas, Wardle, Fiona
Keywords
none
Datasets
GEO:GSE10883
MeSH Terms
  • Mutation/genetics
  • Animals
  • Organ Specificity
  • Nuclear Proteins/metabolism*
  • Muscle Fibers, Fast-Twitch/cytology
  • Transcription, Genetic*
  • Cardiac Myosins/genetics
  • Cell Differentiation
  • Gene Expression Regulation, Developmental
  • Promoter Regions, Genetic/genetics
  • Repressor Proteins/metabolism*
  • Binding Sites
  • Up-Regulation/genetics
  • Chromatin Immunoprecipitation
  • Embryo, Nonmammalian/metabolism*
  • Muscle Fibers, Skeletal/cytology
  • Muscle Fibers, Skeletal/metabolism*
  • Zebrafish Proteins/metabolism*
  • DNA-Binding Proteins/metabolism*
  • Muscle Fibers, Slow-Twitch/cytology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Myosin Light Chains/genetics
(all 23)
PubMed
18535625 Full text @ EMBO Rep.
Abstract
The zebrafish u-boot (ubo) gene encodes the transcription factor Prdm1, which is essential for the specification of the primary slow-twitch muscle fibres that derive from adaxial cells. Here, we show that Prdm1 functions by acting as a transcriptional repressor and that slow-twitch-specific muscle gene expression is activated by Prdm1-mediated repression of the transcriptional repressor Sox6. Genes encoding fast-specific isoforms of sarcomeric proteins are ectopically expressed in the adaxial cells of ubo(tp39) mutant embryos. By using chromatin immunoprecipitation, we show that these are direct targets of Prdm1. Thus, Prdm1 promotes slow-twitch fibre differentiation by acting as a global repressor of fast-fibre-specific genes, as well as by abrogating the repression of slow-fibre-specific genes.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    		Point Mutation
    i106TgTransgenic Insertion
      i111TgTransgenic Insertion
        i130TgTransgenic Insertion
          i135TgTransgenic Insertion
            tp39
              		Point Mutation
              zf13TgTransgenic Insertion
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                prdm1aMO2-prdm1aMRPHLNO
                sox6MO1-sox6MRPHLNO
                1 - 2 of 2
                Show
                Fish
                Fish
                i106Tg
                i106Tg + MO1-sox6
                i111Tg
                i111Tg + MO1-sox6
                i130Tg
                i130Tg + MO2-prdm1a
                i135Tg
                i135Tg + MO2-prdm1a
                prdm1atp39/tp39
                prdm1atp39/tp39; i111Tg
                1 - 10 of 17
                Show
                Antibodies
                Name Type Antigen Genes Isotypes Host Organism
                Ab-F310monoclonal
                  		IgG1Mouse
                  Ab-S58monoclonal
                    		IgAMouse
                    1 - 2 of 2
                    Show
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    EGFPEFGEGFP
                    GFPEFGGFP
                    1 - 2 of 2
                    Show
                    Mapping
                    No data available
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                    Citation
                    von Hofsten, J., Elworthy, S., Gilchrist, M.J., Smith, J.C., Wardle, F.C., and Ingham, P.W. (2008) Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo. EMBO reports. 9(7):683-689.