PUBLICATION

Mandibular arch muscle identity is regulated by a conserved molecular process during vertebrate development

Authors
Knight, R.D., Mebus, K., and Roehl, H.H.
ID
ZDB-PUB-080326-11
Date
2008
Source
Journal of experimental zoology. Part B, Molecular and developmental evolution   310(4): 355-369 (Journal)
Registered Authors
Knight, Robert, Roehl, Henry
Keywords
none
MeSH Terms
  • Body Patterning/physiology*
  • Species Specificity
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Homeodomain Proteins/metabolism
  • Nerve Tissue Proteins/metabolism
  • Fibroblast Growth Factors/metabolism
  • Signal Transduction/physiology
  • Immunohistochemistry
  • In Situ Hybridization
  • Animals
  • Mutation/genetics
  • Mandible/embryology*
  • Muscle, Skeletal/embryology*
(all 14)
PubMed
18338789 Full text @ J. Exp. Zool. B Mol. Dev. Evol.
Abstract
Vertebrate head muscles exhibit a highly conserved pattern of innervation and skeletal connectivity and yet it is unclear whether the molecular basis of their development is likewise conserved. Using the highly conserved expression of Engrailed 2 (En2) as a marker of identity in the dorsal mandibular muscles of zebrafish, we have investigated the molecular signals and tissues required for patterning these muscles. We show that muscle En2 expression is not dependent on signals from the adjacent neural tube, pharyngeal endoderm or axial mesoderm and that early identity of head muscles does not require bone morphogenetic pathway, Notch or Hedgehog (Hh) signalling. However, constrictor dorsalis En2 expression is completely lost after a loss of fibroblast growth factor (Fgf) signalling and we show that is true throughout head muscle development. These results suggest that head muscle identity is dependent on Fgf signalling. Data from experiments performed in chick suggest a similar regulation of En2 genes by Fgf signalling revealing a conserved mechanism for specifying head muscle identity. We present evidence that another key gene important in the development of mouse head muscles, Tbx1, is also critical for specification of mandibular arch muscle identity and that this is independent of Fgf signalling. These data imply that dorsal mandibular arch muscle identity in fish, chick and mouse is specified by a highly conserved molecular process despite differing functions of these muscles in different lineages.
Genes / Markers
Figures
No images available
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cpt_unspecified
    Unspecified
    fgf3_unspecified
      Unspecified
      gli1_unspecified
        Unspecified
        gli2a_unspecified
          Unspecified
          m134
            Point Mutation
            mib1_unspecified
              Unspecified
              noto_unspecified
                Unspecified
                pax2a_unspecified
                  Unspecified
                  smad5_unspecified
                    Unspecified
                    smo_unspecified
                      Unspecified
                      1 - 10 of 13
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Fish
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      No data available
                      Mapping