PUBLICATION

Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development

Authors
Pagnon-Minot, A., Malbouyres, M., Haftek-Terreau, Z., Kim, H.R., Sasaki, T., Thisse, C., Thisse, B., Ingham, P.W., Ruggiero, F., and Le Guellec, D.
ID
ZDB-PUB-080306-3
Date
2008
Source
Developmental Biology   316(1): 21-35 (Journal)
Registered Authors
Ingham, Philip, Kim, Rosemary, Le Guellec, Dominique, Thisse, Bernard, Thisse, Christine
Keywords
Type XV collagen, Extracellular matrix, Connective tissue, Muscle, Development, Zebrafish, Shh, Notochord, Medial fast fiber
MeSH Terms
  • Hedgehog Proteins/metabolism
  • Amino Acid Sequence
  • Base Sequence
  • Animals
  • Collagen/antagonists & inhibitors
  • Collagen/genetics
  • Collagen/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Basement Membrane/embryology
  • Basement Membrane/metabolism
  • Basement Membrane/ultrastructure
  • Muscle Development*/genetics
  • Signal Transduction
  • Body Patterning/genetics
  • Molecular Sequence Data
  • Cloning, Molecular
  • Motor Neurons/physiology
  • Notochord/chemistry
  • Notochord/embryology*
  • Notochord/metabolism
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 24)
PubMed
18281032 Full text @ Dev. Biol.
Abstract
Muscle cells are surrounded by extracellular matrix, the components of which play an important role in signalling mechanisms involved in their development. In mice, loss of collagen XV, a component of basement membranes expressed primarily in skeletal muscles, results in a mild skeletal myopathy. We have determined the complete zebrafish collagen XV primary sequence and analysed its expression and function in embryogenesis. During the segmentation period, expression of the Col15a1 gene is mainly found in the notochord and its protein product is deposited exclusively in the peri-notochordal basement membrane. Morpholino mediated knock-down of Col15a1 causes defects in notochord differentiation and in fast and slow muscle formation as shown by persistence of axial mesodermal marker gene expression, disorganization of the peri-notochodal basement membrane and myofibrils, and a U-shape myotome. In addition, the number of medial fast-twitch muscle fibers was substantially increased, suggesting that the signalling by notochord derived Hh proteins is enhanced by loss of collagen XV. Consistent with this, there is a concomitant expansion of patched-1 expression in the myotome of morphant embryos. Together, these results indicate that collagen XV is required for notochord differentiation and muscle development in the zebrafish embryo and that it interplays with Shh signalling.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-col15a1astandard conditionsPrim-5
WT + MO1-col15a1astandard conditionsPrim-5
WT + MO1-col15a1astandard conditionsPrim-5
WT + MO1-col15a1astandard conditionsPrim-5
WT + MO1-col15a1astandard conditionsPrim-5
WT + MO1-col15a1astandard conditionsPrim-5
WT + MO1-col15a1astandard conditionsLong-pec
WT + MO1-col15a1astandard conditionsLong-pec
WT + MO1-col15a1astandard conditionsLong-pec
WT + MO1-col15a1astandard conditionsLong-pec
1 - 10 of 22
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
i108TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    col15a1aMO1-col15a1aMRPHLNO
    col15a1aMO2-col15a1aMRPHLNO
    1 - 2 of 2
    Show
    Fish
    Fish
    i108Tg
    i108Tg + MO1-col15a1a
    WT
    WT + MO1-col15a1a
    1 - 4 of 4
    Show
    Antibodies
    Orthology
    Gene Orthology
    col15a1a
    1 - 1 of 1
    Show
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    1 - 1 of 1
    Show
    Mapping
    No data available
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    Citation
    Pagnon-Minot, A., Malbouyres, M., Haftek-Terreau, Z., Kim, H.R., Sasaki, T., Thisse, C., Thisse, B., Ingham, P.W., Ruggiero, F., and Le Guellec, D. (2008) Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development. Developmental Biology. 316(1):21-35.