PUBLICATION

Zebrafish homologue irx1a is required for the differentiation of serotonergic neurons

Authors

Cheng, C.W., Yan, C.H., Choy, S.W., Hui, M.N., Hui, C.C., and Cheng, S.H.

ID

ZDB-PUB-070813-18

Date

2007

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 236(9): 2661-2667 (Journal)

Registered Authors

Cheng, Shuk Han

Keywords

5HT, homeodomain, hindbrain, iroquois, zebrafish

MeSH Terms

Homeodomain Proteins/metabolism
Homeodomain Proteins/physiology*
Models, Biological
Developmental Biology/methods
Animals
Serotonin/metabolism
Zebrafish Proteins/metabolism
Zebrafish Proteins/physiology*
Fluorescent Dyes/pharmacology
Apoptosis
In Situ Hybridization
Gene Expression Regulation, Developmental*
Neurons/metabolism
Zebrafish
Neurotransmitter Agents/metabolism
Transcription Factors/metabolism
Transcription Factors/physiology*
Acridine Orange/pharmacology

(all 18)

PubMed

17685478 Full text @ Dev. Dyn.

Abstract

Serotonergic (5HT) neurons produce neurotransmitter serotonin, which modulates various neuronal circuits. The specification and differentiation of 5HT neurons require both extrinsic signals such as Shh and Fgf, as well as intrinsic transcription factors such as nkx2.2, mash1, phox2b, Gata2, and pet1. In this study, we show that iroquois homeodomain factor irx1a, but not irx1b, is expressed in the 5HT neurons. Knockdown of irx1a by antisense morpholino nucleotides reveals that it is a critical determinant for the differentiation of 5HT neurons in the hindbrain. However, irx1a morphants do not show a reduction of the progenitors of 5HT neurons. Hence, irx1a is not required for the initial specification but it is required for the complete differentiation of 5HT neurons.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
gata3	WT	standard conditions	Long-pec	Not Detected	floor plate	ISH	Fig. 2
gata3	WT	standard conditions	Long-pec		hindbrain	ISH	Fig. 2
gata3	WT	control	Prim-15		hindbrain	ISH	Fig. 5
gata3	WT	standard conditions	Long-pec		serotonergic neuron	ISH	Fig. 2
gata3	WT + MO2-irx1a + MO3-irx1a	standard conditions	Prim-15		hindbrain	ISH	Fig. 5
irx1a	WT	standard conditions	5-9 somites		anterior neural rod	ISH	Fig. 1
irx1a	WT	standard conditions	Prim-25		cerebellum	ISH	Fig. 1
irx1a	WT	standard conditions	Prim-5		cerebellum	ISH	Fig. 1
irx1a	WT	standard conditions	Prim-5		hindbrain	ISH	Fig. 1
irx1a	WT	standard conditions	Prim-25		hindbrain	ISH	Fig. 1

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO2-irx1a + MO3-irx1a	standard conditions	Long-pec	hindbrain antero-ventral region apoptotic, abnormal	Fig. 3
WT + MO2-irx1a + MO3-irx1a	standard conditions	Long-pec	hindbrain serotonergic neuron decreased amount, abnormal	Fig. 3

1 - 2 of 2

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
irx1a	MO2-irx1a	MRPHLNO
irx1a	MO3-irx1a	MRPHLNO

Fish

Fish
WT
WT + MO2-irx1a + MO3-irx1a

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Cheng et al., 2007 ZDB-PUB-070813-18 PMID:17685478

Zebrafish homologue irx1a is required for the differentiation of serotonergic neurons

Cheng et al., 2007

ZDB-PUB-070813-18
PMID:17685478