PUBLICATION

Genetic control of single lumen formation in the zebrafish gut

Authors
Bagnat, M., Cheung, I.D., Mostov, K.E., and Stainier, D.Y.
ID
ZDB-PUB-070726-15
Date
2007
Source
Nature cell biology   9(8): 954-960 (Journal)
Registered Authors
Bagnat, Michel, Stainier, Didier
Keywords
none
MeSH Terms
  • Intestines*/abnormalities
  • Intestines*/anatomy & histology
  • Intestines*/embryology
  • Sodium-Potassium-Exchanging ATPase/metabolism
  • Ion Channels/metabolism
  • Hepatocyte Nuclear Factor 1-beta/genetics
  • Hepatocyte Nuclear Factor 1-beta/metabolism*
  • In Situ Hybridization
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Cells, Cultured
  • Ion Transport/physiology
  • Animals, Genetically Modified
  • Membrane Proteins/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Morphogenesis*
  • Molecular Sequence Data
  • Animals
  • Claudins
(all 20)
PubMed
17632505 Full text @ Nat. Cell Biol.
Abstract
Most organs consist of networks of interconnected tubes that serve as conduits to transport fluid and cells and act as physiological barriers between compartments. Biological tubes are assembled through very diverse developmental processes that generate structures of different shapes and sizes. Nevertheless, all biological tubes invariably possess one single lumen. The mechanisms responsible for single lumen specification are not known. Here we show that zebrafish mutants for the MODY5 and familial GCKD gene tcf2 (also known as vhnf1) fail to specify a single lumen in their gut tube and instead develop multiple lumens. We show that Tcf2 controls single lumen formation by regulating claudin15 and Na(+)/K(+)-ATPase expression. Our in vivo and in vitro results indicate that Claudin15 functions in paracellular ion transport to specify single lumen formation. This work shows that single lumen formation is genetically controlled and appears to be driven by the accumulation of fluid.
Genes / Markers
Figures
No images available
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
AB + MO1-cldn15astandard conditionsProtruding-mouth
AB + MO1-cldn15astandard conditionsProtruding-mouth
AB + MO1-oclnastandard conditionsUnknown
epb41l5b781/b781standard conditionsProtruding-mouth
epb41l5b781/b781standard conditionsProtruding-mouth
hnf1bahi2169Tg/hi2169Tgstandard conditionsProtruding-mouth
hnf1bahi2169Tg/hi2169Tgstandard conditionsProtruding-mouth
hnf1bahi2169Tg/hi2169Tgstandard conditionsProtruding-mouth
hnf1bahi2169Tg/hi2169Tgstandard conditionsProtruding-mouth
hnf1bahi2169Tg/hi2169Tgstandard conditionsDay 4
1 - 10 of 18
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b781
    		Point Mutation
    gz4TgTransgenic Insertion
      hi2169TgTransgenic Insertion
      m520
        		Point Mutation
        m567
          		Point Mutation
          s854TgTransgenic Insertion
            1 - 6 of 6
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            cldn15aMO1-cldn15aMRPHLNO
            oclnaMO1-oclnaMRPHLNO
            1 - 2 of 2
            Show
            Fish
            Fish
            AB
            AB + MO1-cldn15a
            AB + MO1-oclna
            epb41l5b781/b781
            hnf1bahi2169Tg/hi2169Tg
            hnf1bahi2169Tg/hi2169Tg; gz4Tg
            pals1am520/m520; s854Tg
            prkcim567/m567; s854Tg
            s854Tg
            1 - 9 of 9
            Show
            Antibodies
            No data available
            Orthology
            Engineered Foreign Genes
            Marker Marker Type Name
            GFPEFGGFP
            1 - 1 of 1
            Show
            Mapping
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            Citation
            Bagnat, M., Cheung, I.D., Mostov, K.E., and Stainier, D.Y. (2007) Genetic control of single lumen formation in the zebrafish gut. Nature cell biology. 9(8):954-960.