PUBLICATION

Daam1 regulates the endocytosis of EphB during the convergent extension of the zebrafish notochord

Authors
Kida, Y.S., Sato, T., Miyasaka, K.Y., Suto, A., and Ogura, T.
ID
ZDB-PUB-070409-26
Date
2007
Source
Proceedings of the National Academy of Sciences of the United States of America   104(16): 6708-6713 (Journal)
Registered Authors
Miyasaka, Kota, Ogura, Toshihiko
Keywords
Daam, Eph, Wnt, planar cell polarity
MeSH Terms
  • Animals
  • Endocytosis/physiology*
  • Humans
  • Cell Adhesion/physiology
  • Receptors, Eph Family/metabolism*
  • Receptors, Eph Family/physiology
  • Cytoskeleton/chemistry
  • Cytoskeleton/metabolism
  • Notochord/chemistry
  • Notochord/embryology*
  • Notochord/metabolism
  • Adaptor Proteins, Signal Transducing/metabolism
  • Adaptor Proteins, Signal Transducing/physiology*
  • Phosphoproteins/metabolism
  • Phosphoproteins/physiology
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
  • Cell Line
  • Subcellular Fractions/chemistry
  • Subcellular Fractions/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism
(all 22)
PubMed
17412835 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Convergent extension (CE) movement of cells is one of the fundamental processes that control the organized morphogenesis of tissues and organs. The molecular events connecting the noncanonical Wnt pathway and CE movement, however, are not well understood. We show that subcellular localization of Daam1, an essential component of noncanonical Wnt signaling, changes dynamically during notochord formation. In the early phases, Daam1 complexes with EphB receptors and Disheveled 2. This complex is incorporated into endocytic vesicles in a dynamin-dependent manner, thereby resulting in the removal of EphB from the cell surface with subsequent switching of cell adhesiveness. In the next step, Daam1 colocalizes with the actin cytoskeleton to induce morphological extension of cells. We elucidate the molecular mechanism underlying the CE movement of notochord cells with Daam1 as a dynamic coordinator of endocytosis and cytoskeletal remodeling.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
daam1aMO2-daam1aMRPHLNO
daam1bMO1-daam1bMRPHLNO
daam2MO1-daam2MRPHLNO
dnm2aMO1-dnm2aMRPHLNO
ephb2aMO1-ephb2aMRPHLNO
ephb4aMO1-ephb4aMRPHLNO
wnt11f2MO1-wnt11f2MRPHLNO
1 - 7 of 7
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Fish
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
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Mapping
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