PUBLICATION

Dynamic somite cell rearrangements lead to distinct waves of myotome growth

Authors
Stellabotte, F., Dobbs-McAuliffe, B., Fernandez, D.A., Feng, X., and Devoto, S.H.
ID
ZDB-PUB-070303-30
Date
2007
Source
Development (Cambridge, England)   134(7): 1253-1257 (Journal)
Registered Authors
Devoto, Stephen Henri, Dobbs-McAuliffe, Betsy, Stellabotte, Frank
Keywords
Myotome, Anterior border cells, Cell fate, Paraxial mesoderm, Somite, Dermomyotome, Zebrafish, Fast muscle, Pax7, Myogenesis, Teleost
MeSH Terms
  • Immunohistochemistry
  • Animals
  • Zebrafish/embryology*
  • Cell Lineage/physiology
  • Myoblasts/physiology*
  • Muscle Development/physiology*
  • Body Weights and Measures
  • Somites/physiology*
  • Microscopy, Confocal
(all 9)
PubMed
17314134 Full text @ Development
Abstract
The myogenic precursors responsible for muscle growth in amniotes develop from the dermomyotome, an epithelium at the external surface of the somite. In teleosts, the myogenic precursors responsible for growth have not been identified. We have used single cell lineage labeling in zebrafish to show that anterior border cells of epithelial somites are myogenic precursors responsible for zebrafish myotome growth. These cells move to the external surface of the embryonic myotome and express the transcription factor Pax7. Some remain on the external surface and some incorporate into the fast myotome, apparently by moving between differentiated slow fibres. The posterior cells of the somite, by contrast, elongate into medial muscle fibres. The surprising movement of the anterior somite cells to the external somite surface transforms a segmentally repeated arrangement of myogenic precursors into a medio-lateral arrangement similar to that seen in amniotes.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
myod1ab1-myod1WTstandard conditions10-13 somitesIHC
myod1ab1-myod1WTstandard conditions10-13 somitesIHC
1 - 2 of 2
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Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
WT
1 - 1 of 1
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Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-myod1polyclonalRabbit
Ab1-prox1
    Ab-MF20monoclonal
      		IgG2bMouse
      Ab-Pax7monoclonal
        		IgG1Mouse
        1 - 4 of 4
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        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available
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        Citation
        Stellabotte, F., Dobbs-McAuliffe, B., Fernandez, D.A., Feng, X., and Devoto, S.H. (2007) Dynamic somite cell rearrangements lead to distinct waves of myotome growth. Development (Cambridge, England). 134(7):1253-1257.