PUBLICATION

Inca: a novel p21-activated kinase-associated protein required for cranial neural crest development

Authors

Luo, T., Xu, Y., Hoffman, T.L., Zhang, T., Schilling, T., and Sargent, T.D.

ID

ZDB-PUB-070303-29

Date

2007

Source

Development (Cambridge, England) 134(7): 1279-1289 (Journal)

Registered Authors

Luo, Ting, Sargent, Tom, Schilling, Tom

Keywords

Cartilage, PAK, Cortical actin, Cytoskeleton, Wound healing, Craniofacial, Tfap2a, Ectomesenchyme, Neural crest, Xenopus, Zebrafish

MeSH Terms

Blotting, Western
Molecular Sequence Data
Anura/embryology
Xenopus/embryology*
Xenopus/metabolism
Immunoprecipitation
In Situ Nick-End Labeling
Animals
In Situ Hybridization
Gene Expression Regulation, Developmental*
Mice
Skull/embryology*
Neural Crest/embryology*
Actins/metabolism
Cell Adhesion/physiology
Yeasts
Xenopus Proteins/genetics*
Xenopus Proteins/metabolism
Zebrafish/embryology
Transcription Factor AP-2/metabolism
Cell Movement/physiology
Microarray Analysis
Amino Acid Sequence
p21-Activated Kinases/metabolism
Nerve Tissue Proteins/genetics*
Nerve Tissue Proteins/metabolism
Oligonucleotides

(all 27)

PubMed

17314132 Full text @ Development

Abstract

Inca (induced in neural crest by AP2) is a novel protein discovered in a microarray screen for genes that are upregulated in Xenopus embryos by the transcriptional activator protein Tfap2a. It has no significant similarity to any known protein, but is conserved among vertebrates. In Xenopus, zebrafish and mouse embryos, Inca is expressed predominantly in the premigratory and migrating neural crest (NC). Knockdown experiments in frog and fish using antisense morpholinos reveal essential functions for Inca in a subset of NC cells that form craniofacial cartilage. Cells lacking Inca migrate successfully but fail to condense into skeletal primordia. Overexpression of Inca disrupts cortical actin and prevents formation of actin 'purse strings', which are required for wound healing in Xenopus embryos. We show that Inca physically interacts with p21-activated kinase 5 (PAK5), a known regulator of the actin cytoskeleton that is co-expressed with Inca in embryonic ectoderm, including in the NC. These results suggest that Inca and PAK5 cooperate in restructuring cytoskeletal organization and in the regulation of cell adhesion in the early embryo and in NC cells during craniofacial development.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
inka1a	tfap2a^{unspecified/unspecified}	standard conditions	10-13 somites	cranial neural crest	ISH	Fig. 3
inka1a	tfap2a^{unspecified/unspecified}	standard conditions	Prim-25	olfactory epithelium	ISH	Fig. 3
inka1a	tfap2a^{unspecified/unspecified}	standard conditions	10-13 somites	pharyngeal arch	ISH	Fig. 3
inka1a	tfap2a^{unspecified/unspecified}	standard conditions	Prim-25	pharyngeal arch	ISH	Fig. 3
inka1a	WT	standard conditions	75%-epiboly	axial chorda mesoderm	ISH	Fig. 2
inka1a	WT	standard conditions	10-13 somites	cranial neural crest	ISH	Fig. 3
inka1a	WT	standard conditions	5-9 somites	cranial neural crest	ISH	Fig. 2
inka1a	WT	standard conditions	Prim-25	diencephalon	ISH	Fig. 2
inka1a	WT	standard conditions	75%-epiboly	ectoderm	ISH	Fig. 2
inka1a	WT	standard conditions	Prim-25	hypophysis	ISH	Fig. 2

1 - 10 of 16

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-inka1a	standard conditions	Day 5	cranial cartilage decreased size, abnormal	Fig. 4
WT + MO1-inka1a	standard conditions	Day 5	head decreased size, abnormal	Fig. 4
WT + MO1-inka1a	standard conditions	Day 5	neurocranium cranial cartilage decreased size, abnormal	Fig. 4
WT + MO1-inka1a	standard conditions	Day 5	pharyngeal arch cranial cartilage decreased size, abnormal	Fig. 4
ba2Tg + MO1-inka1a	standard conditions	Protruding-mouth	cartilage condensation disrupted, abnormal	Fig. 5
ba2Tg + MO1-inka1a	standard conditions	Protruding-mouth	cartilage development disrupted, abnormal	Fig. 5

1 - 6 of 6

Show

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
ba2Tg	Tg(-4.9sox10:EGFP)	Transgenic Insertion
tfap2a_unspecified		Unspecified	tfap2a

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
inka1a	MO1-inka1a	MRPHLNO

Fish

Fish
ba2Tg
ba2Tg + MO1-inka1a
tfap2a^{unspecified/unspecified}
WT
WT + MO1-inka1a

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Luo et al., 2007 ZDB-PUB-070303-29 PMID:17314132

Inca: a novel p21-activated kinase-associated protein required for cranial neural crest development

Luo et al., 2007

ZDB-PUB-070303-29
PMID:17314132