PUBLICATION

Signals and myogenic regulatory factors restrict pax3 and pax7 expression to dermomyotome-like tissue in zebrafish

Authors
Hammond, C.L., Hinits, Y., Osborn, D.P., Minchin, J.E., Tettamanti, G., and Hughes, S.M.
ID
ZDB-PUB-061116-7
Date
2007
Source
Developmental Biology   302(2): 504-521 (Journal)
Registered Authors
Hammond, Chrissy, Hinits, Yaniv, Hughes, Simon M., Minchin, James, Osborn, Dan
Keywords
Pax3, Pax7, Muscle, Zebrafish, Hedgehog, Somite, Fgf8, Dermomyotome, Myf5, Myod, Col1a2, Dlx2a, Sox10
MeSH Terms
  • MyoD Protein/physiology*
  • Paired Box Transcription Factors/biosynthesis*
  • Paired Box Transcription Factors/genetics
  • Muscle Fibers, Skeletal/physiology
  • Gene Expression Regulation, Developmental
  • Muscle Development
  • Embryo, Nonmammalian
  • Somites/physiology
  • Signal Transduction
  • Cell Proliferation
  • PAX7 Transcription Factor/biosynthesis*
  • PAX7 Transcription Factor/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Mutation
  • Myogenic Regulatory Factor 5/physiology*
  • Animals
  • Fibroblast Growth Factors/physiology*
  • Neural Crest/embryology
  • Neural Crest/metabolism
  • Hedgehog Proteins/physiology*
(all 25)
PubMed
17094960 Full text @ Dev. Biol.
Abstract
Pax3/7 paired homeodomain transcription factors are important markers of muscle stem cells. Pax3 is required upstream of myod for lateral dermomyotomal cells in the amniote somite to form particular muscle cells. Later Pax3/7-dependent cells generate satellite cells and most body muscle. Here we analyse early myogenesis from, and regulation of, a population of Pax3-expressing dermomyotome-like cells in the zebrafish. Zebrafish pax3 is widely expressed in the lateral somite and, along with pax7, becomes restricted anteriorly and then to the external cells on the lateral somite surface. Midline-derived Hedgehog signals appear to act directly on lateral somite cells to repress Pax3/7. Both Hedgehog and Fgf8, signals that induce muscle formation within the somite, suppress Pax3/7 and promote expression of myogenic regulatory factors (MRFs) myf5 and myod in specific muscle precursor cell populations. Loss of MRF function leads to loss of myogenesis by specific populations of muscle fibres, with parallel up-regulation of Pax3/7. Myod is required for lateral fast muscle differentiation from pax3-expressing cells. In contrast, either Myf5 or Myod is sufficient to promote slow muscle formation from adaxial cells. Thus, myogenic signals act to drive somite cells to a myogenic fate through up-regulation of distinct combinations of MRFs. Our data show that the relationship between Pax3/7 genes and myogenesis is evolutionarily ancient, but that changes in the MRF targets for particular signals contribute to myogenic differences between species.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-myod1 + MO3-myf5standard conditionsPrim-5
1 - 1 of 1
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    		Point Mutation
    m618
      		Point Mutation
      tbx392
        		Point Mutation
        ti282a
          		Point Mutation
          tp39
            		Point Mutation
            zf13TgTransgenic Insertion
              1 - 6 of 6
              Show
              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              fgf8aMO3-fgf8aMRPHLNO
              myf5MO3-myf5MRPHLNO
              myod1MO1-myod1MRPHLNO
              1 - 3 of 3
              Show
              Fish
              Fish
              fgf8ati282a/ti282a
              prdm1atp39/tp39
              shhatbx392/tbx392
              smob641/b641
              sox10m618/m618
              WT
              WT + MO1-myod1
              WT + MO1-myod1 + MO3-myf5
              WT + MO3-fgf8a
              WT + MO3-myf5
              1 - 10 of 11
              Show
              Antibodies
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              GFPEFGGFP
              1 - 1 of 1
              Show
              Mapping
              No data available
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              Citation
              Hammond, C.L., Hinits, Y., Osborn, D.P., Minchin, J.E., Tettamanti, G., and Hughes, S.M. (2007) Signals and myogenic regulatory factors restrict pax3 and pax7 expression to dermomyotome-like tissue in zebrafish. Developmental Biology. 302(2):504-521.