PUBLICATION

Tbx16 cooperates with Wnt11 in assembling the zebrafish organizer

Authors
Muyskens, J.B., and Kimmel, C.B.
ID
ZDB-PUB-061108-10
Date
2007
Source
Mechanisms of Development   124(1): 35-42 (Journal)
Registered Authors
Kimmel, Charles B., Muyskens, Jon
Keywords
Gastrulation, T-box transcription factors, Planar cell polarity, Live imaging, Protocadherin
MeSH Terms
  • Wnt Proteins/deficiency
  • Wnt Proteins/genetics
  • Wnt Proteins/metabolism*
  • Animals
  • Base Sequence
  • Signal Transduction
  • Gene Expression Regulation, Developmental
  • Cadherins/genetics
  • Cadherins/metabolism
  • Body Patterning
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Organizers, Embryonic/embryology*
  • Organizers, Embryonic/metabolism
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Oligodeoxyribonucleotides, Antisense/genetics
  • Oligodeoxyribonucleotides, Antisense/pharmacology
  • T-Box Domain Proteins/deficiency
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism*
(all 25)
PubMed
17081734 Full text @ Mech. Dev.
Abstract
The organizer, the signaling center that specifies vertebrate axial polarity and the nervous system, is a dorsal midline mesodermal domain in the gastrula that will form prechordal plate and anterior notochord. We show that in zebrafish the organizer is not a single domain when it first arises in the nascent mesoderm at the onset of gastrulation. Rather, in the presumptive prechordal plate region, the organizer is subdivided into two side-by-side cellular fields. Within minutes, concurrent medial and anterior cellular movements merge, or 'coalesce', the two fields to form the well-known singular midline field. Coalescence forms a symmetrical domain because the cell movements on the left and right sides initiate simultaneously and occur synchronously. However, in embryos with reduced function of the T-box transcription factor Tbx16 (Spadetail) or its genetic target paraxial protocadherin (Papc), synchrony is lost, coalesence is disrupted, and the midline domain is misshaped. Furthermore, with combined loss of Tbx16 and Wnt11 (Silberblick), coalesence is essentially absent. Possibly as a consequence, both the anterior movement of presumptive prechordal plate and organizer function, as assayed by eye-field separation, are disrupted. Our findings thus reveal that Tbx16, in combination with Wnt11, are critical components not only in morphogenesis but also in initial assembly of the organizer.
Genes / Markers
Figures
No images available
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    Indel
    tz216
      Point Mutation
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      tbx16MO1-tbx16MRPHLNO
      tbx16MO2-tbx16MRPHLNO
      wnt11f2MO3-wnt11f2MRPHLNO
      1 - 3 of 3
      Show
      Fish
      Antibodies
      No data available
      Orthology
      Engineered Foreign Genes
      No data available
      Mapping