PUBLICATION

The transcription factor ZBP-89 controls generation of the hematopoietic lineage in zebrafish and mouse embryonic stem cells

Authors
Li, X., Xiong, J.W., Shelley, C.S., Park, H., and Arnaout, M.A.
ID
ZDB-PUB-060825-6
Date
2006
Source
Development (Cambridge, England)   133(18): 3641-3650 (Journal)
Registered Authors
Xiong, Jing-Wei
Keywords
Hematopoiesis, Angiogenesis, ZBP-89 gene, SCL/tal-1 gene, Stem cells
MeSH Terms
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology
  • Gene Expression Regulation, Developmental/genetics
  • Humans
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • DNA-Binding Proteins/physiology
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/metabolism
  • Hematopoietic Stem Cells/physiology
  • Mutation/genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Animals
  • Cells, Cultured
  • Flow Cytometry
  • In Situ Hybridization
  • Neovascularization, Physiologic/genetics
  • Mice
  • Blotting, Western
  • In Situ Nick-End Labeling
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Transcription Factors/physiology
  • Hematopoiesis/genetics
  • Hematopoiesis/physiology
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
(all 31)
PubMed
16914492 Full text @ Development
Abstract
Hematopoietic development is closely linked to that of blood vessels and the two processes are regulated in large part by transcription factors that control cell fate decisions and cellular differentiation. Both blood and blood vessels derive from a common progenitor, termed the hemangioblast, but the factor(s) specifying the development and differentiation of this stem cell population into the hematopoietic and vascular lineages remain ill defined. Here, we report that knockdown of the Kruppel-like transcription factor ZBP-89 in zebrafish embryos results in a bloodless phenotype, caused by disruption of both primitive and definitive hematopoiesis, while leaving primary blood vessel formation intact. Injection of ZBP-89 mRNA into cloche zebrafish embryos, which lack both the hematopoietic and endothelial lineages, rescues hematopoiesis but not vasculogenesis. Injection of mRNA for Stem Cell Leukemia (SCL), a transcription factor that directs hemangioblast development into blood cell precursors, rescues the bloodless phenotype in ZBP-89 zebrafish morphants. Forced expression of ZBP-89 induces the expansion of hematopoietic progenitors in wild-type zebrafish and in mouse embryonic stem cell cultures but inhibits angiogenesis in vivo and in vitro. These findings establish a unique regulatory role for ZBP-89, positioned at the interface between early blood and blood vessel development.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
la781TgTransgenic Insertion
    m39
      Deficiency
      y1TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        znf148MO1-znf148MRPHLNO
        znf148MO2-znf148MRPHLNO
        1 - 2 of 2
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        Fish
        Antibodies
        No data available
        Orthology
        Gene Orthology
        znf148
        1 - 1 of 1
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        Engineered Foreign Genes
        No data available
        Mapping
        No data available