PUBLICATION

Prdm1 acts downstream of a sequential RA, Wnt and Fgf signaling cascade during zebrafish forelimb induction

Authors

Mercader, N., Fischer, S., and Neumann, C.J.

ID

ZDB-PUB-060623-25

Date

2006

Source

Development (Cambridge, England) 133(15): 2805-2815 (Journal)

Registered Authors

Neumann, Carl J.

Keywords

Limb development, Prdm1, Tbx5, Retinoic acid, Fgf, Wnt2b

MeSH Terms

Animals
Zebrafish Proteins/physiology
Tretinoin/physiology*
Mesoderm/physiology*
Wnt Proteins/physiology*
Repressor Proteins/physiology*
Zebrafish/embryology*
Morphogenesis
Limb Buds/physiology*
Mutation
Fibroblast Growth Factors/physiology*
DNA-Binding Proteins
Glycoproteins/physiology
Signal Transduction
Embryo, Nonmammalian/physiology
Forelimb/embryology*
Nuclear Proteins
Transcription Factors/physiology*

(all 18)

PubMed

16790478 Full text @ Development

CTD

16790478

Abstract

Vertebrate limb induction is triggered in the lateral plate mesoderm (LPM) by a cascade of signaling events originating in the axial mesoderm. While it is known that Fgf, Wnt and retinoic acid (RA) signals are involved in this cascade, their precise regulatory hierarchy has not been determined in any species. tbx5 is the earliest gene expressed in the limb bud mesenchyme. Recently, another transcription factor, Prdm1, has been shown to be crucial for zebrafish forelimb development. Here, we show that Prdm1 is downstream of RA, Wnt2b and Tbx5 activity. We find that RA activity, but not Fgf signaling, is necessary for wnt2b expression. Fgf signaling is required for prdm1 expression in the fin bud, but is not necessary for the initiation of tbx5 expression. We propose a model in which RA signaling from the somitic mesoderm leads to activation of wnt2b expression in the intermediate mesoderm, which then signals to the LPM to trigger tbx5 expression. tbx5 is required for Fgf signaling in the limb bud leading to activation of prdm1 expression, which in turn is required for downstream activation of fgf10 expression.

Genes / Markers

Figures