PUBLICATION

oko meduzy and Related crumbs Genes Are Determinants of Apical Cell Features in the Vertebrate Embryo

Authors
Omori, Y., and Malicki, J.
ID
ZDB-PUB-060531-9
Date
2006
Source
Current biology : CB   16(10): 945-957 (Journal)
Registered Authors
Malicki, Jarema, Omori, Yoshihiro
Keywords
none
MeSH Terms
  • Nerve Tissue Proteins/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Photoreceptor Cells, Vertebrate/cytology
  • Cell Differentiation/physiology
  • Sequence Homology, Nucleic Acid
  • Membrane Proteins/genetics
  • Membrane Proteins/physiology*
  • Drosophila Proteins/genetics
  • Animals
  • Zebrafish Proteins/physiology*
  • Neuroepithelial Cells/cytology*
  • Cilia/physiology
  • Cilia/ultrastructure
  • Hair Cells, Auditory/embryology
  • Nephrons/embryology
  • Cell Polarity/genetics
  • Cell Polarity/physiology*
  • Genes, Developmental
(all 19)
PubMed
16713951 Full text @ Curr. Biol.
Abstract
BACKGROUND: Polarity is an essential attribute of most eukaryotic cells. One of the most prominent features of cell polarity in many tissues is the subdivision of cell membrane into apical and basolateral compartments by a belt of cell junctions. The proper formation of this subdivision is of key importance. In sensory cells, for example, the apical membrane compartment differentiates specialized structures responsible for the detection of visual, auditory, and olfactory stimuli. In other tissues, apical specializations are responsible for the propagation of fluid flow. Despite its importance, the role of genetic determinants of apico-basal polarity in vertebrate embryogenesis remains poorly investigated. RESULTS: We show that zebrafish oko meduzy (ome) locus encodes a crumbs gene homolog, essential for the proper apico-basal polarity of neural tube epithelia. Two ome paralogs, crb2b and crb3a, promote the formation of apical cell features: photoreceptor inner segments and cilia in renal and auditory systems. The motility of cilia is defective following the impairment of crb2b function. Apical surface defects in ome- and crb2b-deficient animals are associated with profound disorganization of neuronal architecture and with the formation of pronephric cysts, respectively. Unexpectedly, despite differences in their structure and expression patterns, crumbs genes are, at least partially, functionally interchangeable. CONCLUSIONS: ome and related crumbs genes are necessary for the formation of gross morphological features in several organs, including the CNS and the renal system. On the cellular level, crumbs genes regulate the formation of both ciliary and nonciliary apical membrane compartment.
Genes / Markers
Figures
No images available
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b781
    		Point Mutation
    m98
      		Point Mutation
      m227
        		Point Mutation
        m289
          		Point Mutation
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          crb2aMO1-crb2aMRPHLNO
          crb2aMO2-crb2aMRPHLNO
          crb2bMO1-crb2bMRPHLNO
          crb2bMO2-crb2bMRPHLNO
          crb3aMO1-crb3aMRPHLNO
          crb3aMO2-crb3aMRPHLNO
          1 - 6 of 6
          Show
          Fish
          Fish
          crb2am98/m98 (AB)
          crb2am289/m289 (AB)
          epb41l5b781/b781
          pals1am227/m227 (AB)
          WT
          1 - 5 of 5
          Show
          Antibodies
          Orthology
          Engineered Foreign Genes
          No data available
          Mapping
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          Citation
          Omori, Y., and Malicki, J. (2006) oko meduzy and Related crumbs Genes Are Determinants of Apical Cell Features in the Vertebrate Embryo. Current biology : CB. 16(10):945-957.