PUBLICATION

Zebrafish Trap230/Med12 is required as a coactivator for Sox9-dependent neural crest, cartilage and ear development

Authors
Rau, M.J., Fischer, S., and Neumann, C.J.
ID
ZDB-PUB-060531-8
Date
2006
Source
Developmental Biology   296(1): 83-93 (Journal)
Registered Authors
Neumann, Carl J., Rau, Marlene J.
Keywords
Zebrafish, Development, Neural crest, Limb, Mediator, TRAP230, MED12, Sox9, Chondrogenesis, Pectoral fins, Trapped
MeSH Terms
  • Neural Crest/embryology*
  • Mediator Complex
  • High Mobility Group Proteins/physiology*
  • Phenotype
  • Zebrafish/embryology*
  • SOX9 Transcription Factor
  • Receptors, Thyroid Hormone/genetics
  • Receptors, Thyroid Hormone/physiology*
  • Animals
  • Ear/embryology*
  • Cartilage/embryology*
  • HMGB Proteins/physiology*
  • Protein Subunits/genetics
  • Protein Subunits/physiology
  • Trans-Activators/genetics
  • Trans-Activators/physiology
  • Transcription Factors/genetics
  • Transcription Factors/physiology*
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
(all 22)
PubMed
16712834 Full text @ Dev. Biol.
Abstract
The vertebrate Sox9 transcription factor directs the development of neural crest, otic placodes, cartilage and bone. In zebrafish, there are two Sox9 orthologs, Sox9a and Sox9b, which together perform the functions of the single-copy tetrapod Sox9. In a large-scale genetic screen, we have identified a novel zebrafish mutant that strongly resembles the Sox9a/Sox9b double mutant phenotype. We show that this mutation disrupts the zebrafish Trap230/Med12 ortholog, a member of the Mediator complex. Mediator is a coactivator complex transducing the interaction of DNA-binding transcription factors with RNA polymerase II, and our results reveal a critical function of the Trap230 subunit as a coactivator for Sox9.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
t25870
    		Point Mutation
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    med12MO2-med12MRPHLNO
    1 - 1 of 1
    Show
    Fish
    Fish
    med12t25870/t25870
    WT
    WT + MO2-med12
    1 - 3 of 3
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    Entity Type Entity Symbol Location
    GENEmed12Chr: 14 Details
    SSLPz11725Chr: 14 Details
    SSLPz53264Chr: 14 Details
    1 - 3 of 3
    Show
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    Citation
    Rau, M.J., Fischer, S., and Neumann, C.J. (2006) Zebrafish Trap230/Med12 is required as a coactivator for Sox9-dependent neural crest, cartilage and ear development. Developmental Biology. 296(1):83-93.