PUBLICATION

MyD88 Innate Immune Function in a Zebrafish Embryo Infection Model

Authors
van der Sar, A.M., Stockhammer, O.W., van der Laan, C., Spaink, H.P., Bitter, W., and Meijer, A.H.
ID
ZDB-PUB-060323-32
Date
2006
Source
Infection and Immunity   74(4): 2436-2441 (Journal)
Registered Authors
Meijer, Annemarie H., Spaink, Herman P.
Keywords
none
MeSH Terms
  • Disease Models, Animal
  • Salmonella Infections/genetics
  • Salmonella Infections/immunology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Immunity, Innate*/genetics
  • Salmonella typhimurium/genetics
  • Salmonella typhimurium/immunology
  • Oligonucleotides, Antisense/metabolism
  • Signal Transduction/genetics
  • Signal Transduction/immunology*
  • Bacterial Outer Membrane Proteins/biosynthesis*
  • Bacterial Outer Membrane Proteins/genetics
  • Bacterial Outer Membrane Proteins/metabolism
  • Myeloid Differentiation Factor 88
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/physiology*
  • Molecular Sequence Data
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/immunology*
  • Animals
  • Toll-Like Receptors/genetics
  • Toll-Like Receptors/physiology
(all 23)
PubMed
16552074 Full text @ Infect. Immun.
Abstract
Innate immunity signaling mechanisms during vertebrate embryogenesis are largely unknown. To study Toll-like receptor (TLR) signaling function in the zebrafish embryo model, we designed an experimental setup for antisense morpholino knockdown under conditions of bacterial infection. Clearance of Salmonella enterica serovar Typhimurium Ra bacteria was significantly impaired after knockdown of myeloid differentiation factor 88 (MyD88), a common adaptor protein in TLR and interleukin-1 receptor signaling. Thereby, we demonstrate for the first time that the innate immune response of the developing embryo involves MyD88-dependent signaling, which further establishes the zebrafish embryo as a model for the study of vertebrate innate immunity.
Genes / Markers
Figures
No images available
Show all Figures
Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
lcp1WT + MO1-myd88standard conditionsPrim-5ISH
mpxWT + MO1-myd88standard conditionsLong-pecIHC
mpxWT + MO1-myd88physical alteration: caudal finLong-pecIHC
myd88WTstandard conditions4-cell to AdultRTPCR
sarm1WTstandard conditions4-cell to AdultRTPCR
ticam1WTstandard conditions4-cell to AdultRTPCR
tirapWTstandard conditions4-cell to AdultRTPCR
tlr1WTstandard conditionsDome to 5-9 somitesRTPCR
tlr18WTstandard conditions4-cell to AdultRTPCR
tlr2WTstandard conditions4-cell to AdultRTPCR
1 - 10 of 22
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Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
myd88MO1-myd88MRPHLNO
1 - 1 of 1
Show
Fish
Fish
WT
WT + MO1-myd88
1 - 2 of 2
Show
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
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Citation
van der Sar, A.M., Stockhammer, O.W., van der Laan, C., Spaink, H.P., Bitter, W., and Meijer, A.H. (2006) MyD88 Innate Immune Function in a Zebrafish Embryo Infection Model. Infection and Immunity. 74(4):2436-2441.