PUBLICATION

Specification of the Primitive Myeloid Precursor Pool Requires Signaling through Alk8 in Zebrafish

Authors
Hogan, B.M., Layton, J.E., Pyati, U.J., Nutt, S.L., Hayman, J.W., Varma, S., Heath, J.K., Kimelman, D., and Lieschke, G.J.
ID
ZDB-PUB-060315-18
Date
2006
Source
Current biology : CB   16(5): 506-511 (Journal)
Registered Authors
Hayman, John W., Heath, Joan K., Hogan, Ben M., Kimelman, David, Layton, Judy E., Lieschke, Graham J., Pyati, Ujwal, Varma, Sony
Keywords
none
MeSH Terms
  • Cells, Cultured
  • Mice
  • Bone Morphogenetic Protein Receptors/metabolism
  • Models, Biological
  • Gene Expression Regulation, Developmental
  • Activin Receptors, Type I/genetics
  • Activin Receptors, Type I/metabolism
  • Activin Receptors, Type I/physiology*
  • Animals
  • Cell Differentiation
  • Body Patterning
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Myeloid Progenitor Cells/cytology
  • Myeloid Progenitor Cells/metabolism*
  • Myelopoiesis*
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/metabolism
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
  • Signal Transduction*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
(all 24)
PubMed
16527746 Full text @ Curr. Biol.
Abstract
In the zebrafish embryo, primitive hematopoiesis initiates in two spatially distinct regions. Rostrally, the cells of the anterior lateral plate mesoderm (ALPM) give rise exclusively to cells of the myeloid lineage in a pu.1-dependent manner . Caudally, in the posterior lateral plate mesoderm (PLPM), the expression of gata1 defines a precursor pool that gives rise predominantly to the embryonic erythrocytes . The transcription factor scl acts upstream of both gata1 and pu.1 in these precursor pools, activating a series of conserved transcription factors that cell-autonomously specify either myeloid or erythroid fates . However, the mechanisms underlying the spatial separation of the hematopoietic precursor pools and the induction of differential gene expression within these pools are not well understood. We show here that the Bmp receptor lost-a-fin/alk8 is required for rostral pu.1 expression and myelopoiesis, identifying an early genetic event that distinguishes between the induction of anterior and posterior hematopoiesis. Introducing a constitutively active version of the Alk8 receptor led to increased pu.1 expression, but the role of alk8 was independent of the scl-dependent cell-fate pathway. Furthermore, the role of Alk8 in myelopoiesis was genetically separable from its earlier role in dorsal-ventral embryonic patterning.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
cdh17acvr1lgl2/gl2standard conditionsPrim-5ISH
cdh17WTstandard conditionsPrim-5ISH
fli1acvr1lgl2/gl2standard conditions14-19 somitesISH
fli1WTstandard conditions14-19 somitesISH
gata1aacvr1lgl2/gl2standard conditions14-19 somitesNot DetectedISH
gata1aacvr1lgl2/gl2standard conditions14-19 somites to 20-25 somitesISH
gata1aWTstandard conditions14-19 somitesNot DetectedISH
gata1aWTstandard conditions14-19 somites to 20-25 somitesISH
hbae3acvr1lgl2/gl2standard conditions14-19 somites to 20-25 somitesISH
hbae3WTstandard conditions14-19 somites to 20-25 somitesISH
1 - 10 of 34
Show
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
gl2
    		Point Mutation
    m39
      		Deficiency
      tm110b
        		Point Mutation
        w30TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          admpMO1-admpMRPHLNO
          bmp2bMO2-bmp2bMRPHLNO
          bmp4MO1-bmp4MRPHLNO
          bmp7aMO2-bmp7aMRPHLNO
          gdf6aMO1-gdf6aMRPHLNO
          1 - 5 of 5
          Show
          Fish
          Fish
          acvr1lgl2/gl2
          acvr1ltm110b/tm110b (TU)
          Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39 (INDO)
          w30Tg (WIK/AB)
          WT
          1 - 5 of 5
          Show
          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          No data available
          Mapping
          Entity Type Entity Symbol Location
          Featuregl2Chr: 2 Details
          SSLPz1406Chr: 2 Details
          1 - 2 of 2
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          Citation
          Hogan, B.M., Layton, J.E., Pyati, U.J., Nutt, S.L., Hayman, J.W., Varma, S., Heath, J.K., Kimelman, D., and Lieschke, G.J. (2006) Specification of the Primitive Myeloid Precursor Pool Requires Signaling through Alk8 in Zebrafish. Current biology : CB. 16(5):506-511.