PUBLICATION

belladonna/(lhx2) is required for neural patterning and midline axon guidance in the zebrafish forebrain

Authors
Seth, A., Culverwell, J., Walkowicz, M., Toro, S., Rick, J.M., Neuhauss, S.C., Varga, Z.M., and Karlstrom, R.O.
ID
ZDB-PUB-060130-7
Date
2006
Source
Development (Cambridge, England)   133(4): 725-735 (Journal)
Registered Authors
Karlstrom, Rolf, Neuhauss, Stephan, Rick, Jens, Seth, Anandita, Toro, Sabrina, Varga, Zoltán M., Walkowicz, Mitch
Keywords
AC, Chiasm, Commissure, Ephrin, Gfap, Glial bridge, Morpholino, Netrin, POC, Semaphorin, Slit
MeSH Terms
  • LIM-Homeodomain Proteins
  • Neuroglia/physiology
  • Amino Acid Sequence
  • Eye/cytology
  • Eye/embryology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Animals
  • Transcription Factors
  • Axons/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Molecular Sequence Data
  • Signal Transduction
  • Cell Proliferation
  • Prosencephalon/embryology*
  • Body Patterning*
  • Mutation
  • Diencephalon/embryology
  • Diencephalon/metabolism
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology
  • Telencephalon/embryology
  • Telencephalon/metabolism
  • Fibroblast Growth Factors/metabolism
  • Morphogenesis
(all 27)
PubMed
16436624 Full text @ Development
Abstract
Some of the earliest axon pathways to form in the vertebrate forebrain are established as commissural and retinal axons cross the midline of the diencephalon and telencephalon. To better understand axon guidance in the forebrain, we characterized the zebrafish belladonna (bel) mutation, which disrupts commissural and retinal axon guidance in the forebrain. Using a positional cloning strategy, we determined that the bel locus encodes zebrafish Lhx2, a lim-homeodomain transcription factor expressed in the brain, eye and fin buds. We show that bel(lhx2) function is required for patterning in the ventral forebrain and eye, and that loss of bel function leads to alterations in regulatory gene expression, perturbations in axon guidance factors, and the absence of an optic chiasm and forebrain commissures. Our analysis reveals new roles for lhx2 in midline axon guidance, forebrain patterning and eye morphogenesis.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
efnb2alhx2bunspecifiedcontrolPrim-5ISH
efnb2alhx2bunspecifiedcontrolPrim-5ISH
efnb2aWTcontrolPrim-5ISH
efnb2aWTcontrolPrim-5ISH
etv5bWTchemical treatment: SU5402BudNot DetectedISH
etv5bWTcontrolBudISH
fgf8alhx2bunspecifiedcontrolPrim-5ISH
fgf8aWTcontrolPrim-5ISH
gfaplhx2bunspecifiedstandard conditionsPrim-5IHC
gfaplhx2bunspecifiedstandard conditions20-25 somitesIHC
1 - 10 of 61
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b700
    		Small Deletion
    lhx2b_unspecified
      		Unspecified
      tv42z
        		Point Mutation
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        lhx2bMO2-lhx2bMRPHLNO
        1 - 1 of 1
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        Fish
        Fish
        lhx2bb700/b700 (AB, TL)
        lhx2btv42z/tv42z
        lhx2bunspecified
        WT
        WT + MO2-lhx2b
        1 - 5 of 5
        Show
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        Entity Type Entity Symbol Location
        GENElhx2bChr: 8 Details
        SSLPz24272Chr: 8 Details
        SSLPz44909Chr: 8 Details
        1 - 3 of 3
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        Citation
        Seth, A., Culverwell, J., Walkowicz, M., Toro, S., Rick, J.M., Neuhauss, S.C., Varga, Z.M., and Karlstrom, R.O. (2006) belladonna/(lhx2) is required for neural patterning and midline axon guidance in the zebrafish forebrain. Development (Cambridge, England). 133(4):725-735.