PUBLICATION

Influences on neural lineage and mode of division in the zebrafish retina in vivo

Authors
Poggi, L., Vitorino, M., Masai, I., and Harris, W.A.
ID
ZDB-PUB-051221-7
Date
2005
Source
The Journal of cell biology   171(6): 991-999 (Journal)
Registered Authors
Harris, William A., Masai, Ichiro, Poggi, Lucia
Keywords
none
MeSH Terms
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Nervous System/embryology
  • Nervous System/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Animals
  • Cell Lineage*
  • Retinal Ganglion Cells/metabolism
  • Animals, Genetically Modified
  • Retina/embryology*
  • Retina/metabolism
  • Cell Division/genetics
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Mitosis
  • Microscopy, Video
  • Cell Differentiation
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
(all 20)
PubMed
16365165 Full text @ J. Cell Biol.
Abstract
Cell determination in the retina has been under intense investigation since the discovery that retinal progenitors generate clones of apparently random composition (Price, J., D. Turner, and C. Cepko. 1987. Proc. Natl. Acad. Sci. USA. 84:156-160; Holt, C.E., T.W. Bertsch, H.M. Ellis, and W.A. Harris. 1988. Neuron. 1:15-26; Wetts, R., and S.E. Fraser. 1988. Science. 239:1142-1145). Examination of fixed tissue, however, sheds little light on lineage patterns or on the relationship between the orientation of division and cell fate. In this study, three-dimensional time-lapse analyses were used to trace lineages of retinal progenitors expressing green fluorescent protein under the control of the ath5 promoter. Surprisingly, these cells divide just once along the circumferential axis to produce two postmitotic daughters, one of which becomes a retinal ganglion cell (RGC). Interestingly, when these same progenitors are transplanted into a mutant environment lacking RGCs, they often divide along the central-peripheral axis and produce two RGCs. This study provides the first insight into reproducible lineage patterns of retinal progenitors in vivo and the first evidence that environmental signals influence the orientation of cell division and the lineage of neural progenitors.
Genes / Markers
Figures
No images available
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
rw021TgTransgenic Insertion
    th241
      Point Mutation
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      1 - 2 of 2
      Show
      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      zn-5monoclonalIgG1Mouse
      zpr-1monoclonalIgG1Mouse
      1 - 2 of 2
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      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      1 - 1 of 1
      Show
      Mapping