PUBLICATION
Essential and overlapping roles for laminin alpha chains in notochord and blood vessel formation
- Authors
- Pollard, S.M., Parsons, M.J., Kamei, M., Kettleborough, R.N., Thomas, K.A., Pham, V.N., Bae, M.K., Scott, A., Weinstein, B.M., and Stemple, D.L.
- ID
- ZDB-PUB-051207-18
- Date
- 2006
- Source
- Developmental Biology 289(1): 64-76 (Journal)
- Registered Authors
- Kamei, Makoto, Kettleborough, Ross, Parsons, Michael, Pham, Van, Scott, Annabelle, Stemple, Derek L., Thomas, Kevin, Weinstein, Brant M.
- Keywords
- Laminin, Notochord, Blood vessels, Basement membrane, Cell differentiation
- MeSH Terms
-
- Neovascularization, Physiologic*
- Endothelial Cells/cytology
- Endothelial Cells/physiology
- Notochord/blood supply*
- Notochord/embryology*
- Cell Movement
- RNA, Messenger/analysis
- RNA, Messenger/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Mutation
- Laminin/genetics
- Laminin/physiology*
- Blood Vessels/chemistry
- Blood Vessels/embryology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Animals
- PubMed
- 16321372 Full text @ Dev. Biol.
Citation
Pollard, S.M., Parsons, M.J., Kamei, M., Kettleborough, R.N., Thomas, K.A., Pham, V.N., Bae, M.K., Scott, A., Weinstein, B.M., and Stemple, D.L. (2006) Essential and overlapping roles for laminin alpha chains in notochord and blood vessel formation. Developmental Biology. 289(1):64-76.
Abstract
Laminins are major constituents of basement membranes and have wide ranging functions during development and in the adult. They are a family of heterotrimeric molecules created through association of an alpha, beta and gamma chain. We previously reported that two zebrafish loci, grumpy (gup) and sleepy (sly), encode laminin beta1 and gamma1, which are important both for notochord differentiation and for proper intersegmental blood vessel (ISV) formation. In this study we show that bashful (bal) encodes laminin alpha1 (lama1). Although the strongest allele, bal(m190), is fully penetrant, when compared to gup or sly mutant embryos, bal mutants are not as severely affected, as only anterior notochord fails to differentiate and ISVs are unaffected. This suggests that other alpha chains, and hence other isoforms, act redundantly to laminin 1 in posterior notochord and ISV development. We identified cDNA sequences for lama2, lama4 and lama5 and disrupted the expression of each alone or in mutant embryos also lacking laminin alpha1. When expression of laminin alpha4 and laminin alpha1 are simultaneously disrupted, notochord differentiation and ISVs are as severely affected as sly or gup mutants. Moreover, live imaging of transgenic embryos expressing enhanced green fluorescent protein in forming ISVs reveals that the vascular defects in these embryos are due to an inability of ISV sprouts to migrate correctly along the intersegmental, normally laminin-rich regions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping