PUBLICATION

Patterning of proneuronal and inter-proneuronal domains by hairy- and enhancer of split-related genes in zebrafish neuroectoderm

Authors
Bae, Y.K., Shimizu, T., and Hibi, M.
ID
ZDB-PUB-050221-2
Date
2005
Source
Development (Cambridge, England)   132(6): 1375-1385 (Journal)
Registered Authors
Bae, Young Ki, Hibi, Masahiko, Shimizu, Takashi
Keywords
Neurogenesis, hairy, enhancer-of-split, Proneural gene, Proneuronal domain
MeSH Terms
  • Body Patterning/physiology
  • Animals
  • Membrane Proteins/metabolism
  • Bone Morphogenetic Proteins/metabolism
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Basic Helix-Loop-Helix Transcription Factors
  • Nervous System/metabolism*
  • Ectoderm/metabolism*
  • Zebrafish/embryology*
  • Receptors, Notch
  • Neurons/metabolism
(all 14)
PubMed
15716337 Full text @ Development
Abstract
In teleosts and amphibians, the proneuronal domains, which give rise to primary-motor, primary-inter and Rohon-Beard (RB) neurons, are established at the beginning of neurogenesis as three longitudinal stripes along the anteroposterior axis in the dorsal ectoderm. The proneuronal domains are prefigured by the expression of basic helix-loop-helix (bHLH) proneural genes, and separated by domains (inter-proneuronal domains) that do not express the proneural genes. Little is known about how the formation of these domains is spatially regulated. We have found that the zebrafish hairy- and enhancer of split-related (Her) genes her3 and her9 are expressed in the inter-proneuronal domains, and are required for their formation. her3 and her9 expression was not regulated by Notch signaling, but rather controlled by positional cues, in which Bmp signaling is involved. Inhibition of Her3 or Her9 by antisense morpholino oligonucleotides led to ectopic expression of the proneural genes in part of the inter-proneuronal domains. Combined inhibition of Her3 and Her9 induced ubiquitous expression of proneural and neuronal genes in the neural plate, and abolished the formation of the inter-proneuronal domains. Furthermore, inhibition of Her3/Her9 and Notch signaling led to ubiquitous and homogeneous expression of proneural and neuronal genes in the neural plate, revealing that Her3/Her9 and Notch signaling have distinct roles in neurogenesis. These data indicate that her3 and her9 function as prepattern genes that link the positional dorsoventral polarity information in the posterior neuroectoderm to the spatial regulation of neurogenesis.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m178
    		Point Mutation
    tc300a
      		Point Mutation
      ty68a
        		Point Mutation
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        her3MO1-her3MRPHLNO
        her3MO3-her3MRPHLNO
        her9MO1-her9MRPHLNO
        her9MO2-her9MRPHLNO
        neurog1MO4-neurog1MRPHLNO
        olig2MO1-olig2MRPHLNO
        1 - 6 of 6
        Show
        Fish
        Fish
        bmp2btc300a/tc300a (TU)
        bmp7aty68a/ty68a
        mib1m178/m178 (AB)
        mib1m178/m178 + MO1-her9 (AB)
        mib1m178/m178 + MO1-her9 + MO3-her3 (AB)
        WT
        WT + MO1-her9
        WT + MO1-her9 + MO3-her3
        WT + MO1-olig2 + MO4-neurog1
        WT + MO3-her3
        1 - 10 of 10
        Show
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available
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        Citation
        Bae, Y.K., Shimizu, T., and Hibi, M. (2005) Patterning of proneuronal and inter-proneuronal domains by hairy- and enhancer of split-related genes in zebrafish neuroectoderm. Development (Cambridge, England). 132(6):1375-1385.