PUBLICATION

Zebrafish periostin is required for the adhesion of muscle fiber bundles to the myoseptum and for the differentiation of muscle fibers

Authors
Kudo, H., Amizuka, N., Araki, K., Inohaya, K., and Kudo A.
ID
ZDB-PUB-040312-2
Date
2004
Source
Developmental Biology   267(2): 473-487 (Journal)
Registered Authors
Inohaya, Keiji
Keywords
none
MeSH Terms
  • Molecular Sequence Data
  • Microscopy, Electron
  • Cell Adhesion Molecules/genetics*
  • Cell Adhesion Molecules/metabolism
  • Cell Adhesion Molecules/physiology*
  • Morphogenesis
  • Zebrafish
  • Blotting, Western
  • Animals
  • Enzyme-Linked Immunosorbent Assay
  • Immunohistochemistry
  • Connective Tissue/embryology*
  • Connective Tissue/metabolism
  • Zebrafish Proteins
  • Phylogeny
  • Gene Expression Profiling
  • Somites/ultrastructure
  • In Situ Hybridization
  • Muscle Fibers, Skeletal/cytology
  • Muscle Fibers, Skeletal/metabolism
  • Muscle Fibers, Skeletal/physiology*
  • Amino Acid Sequence
  • Cell Differentiation/physiology*
  • DNA Primers
  • Histological Techniques
  • DNA, Complementary/genetics
(all 26)
PubMed
15013807 Full text @ Dev. Biol.
Abstract
The myoseptum of fishes, composed of dense collagen, is a connective tissue layer that forms in the embryo, dividing somites from the trunk, and its structure and function are similar to those of the mammalian tendon. Both the myoseptum and tendon serve as the transmitter of muscular contractility to bones and adjoining muscles, and their structure is indispensable for movement of vertebrate animals. We cloned the zebrafish periostin gene and examined its expression and function in the myoseptum. The expression in embryos started in the rostral part of each segmented somite in the early segmentation stage; and consequently, metameric stripes were observed. At the end of segmentation, the expression region shifted to the transverse myoseptum and the myotome-epidermis boundary, and each myotome was surrounded by periostin. Using a polyclonal antibody, we found that the periostin protein was localized to the transverse myoseptum. Consistently, periostin morpholino antisense oligonucleotide led to defects in myoseptum formation, a delay in the differentiation of myofibers, and disorder of connection between myofibrils and myoseptum. We demonstrated here that periostin is the first molecule involved in myoseptum formation and propose that periostin secretion on the surface of the myoseptum is required for the adhesion of muscle fiber bundles to the myoseptum and the differentiation of muscle fibers.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ti1
    		Small Deletion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    postnbMO1-postnbMRPHLNO
    1 - 1 of 1
    Show
    Fish
    Fish
    tbx6ti1/ti1
    1 - 1 of 1
    Show
    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab1-postnpolyclonalRabbit
    1 - 1 of 1
    Show
    Orthology
    Gene Orthology
    postnb
    1 - 1 of 1
    Show
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    Kudo, H., Amizuka, N., Araki, K., Inohaya, K., and Kudo A. (2004) Zebrafish periostin is required for the adhesion of muscle fiber bundles to the myoseptum and for the differentiation of muscle fibers. Developmental Biology. 267(2):473-487.